Mutemi Kevin Nzumbi, Simakov Oleg, Pan Leslie, Santangeli Luca, Null Ryan, Handberg-Thorsager Mette, Vellutini Bruno Cossermelli, Peterson Kevin J, Fromm Bastian, Larsson Tomas, Savage Emily, Lopez Mireia Osuna, Hercog Rajna, Provaznik Jan, Ordoñez-Rueda Diana, Azevedo Nayara, Gazave Eve, Vervoort Michel, Tomancak Pavel, Tan Wenhua, Winkler Sylke, Benes Vladimir, Hui Jerome, Helm Conrad, Özpolat B Duygu, Arendt Detlev
European Molecular Biology Laboratory, Developmental Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Department for Neurosciences and Developmental Biology, University of Vienna, Austria.
bioRxiv. 2024 Nov 11:2024.06.21.600153. doi: 10.1101/2024.06.21.600153.
The marine annelid is a model organism used in many research areas including evolution and development, neurobiology, ecology and regeneration. Here we present the genomes of (laboratory culture reference and a single individual assembly) and of the closely related and (single individual assembly) to facilitate comparative genomic approaches and help explore biology. We used long-read sequencing technology and chromosomal-conformation capture along with extensive transcriptomic resources to obtain and annotate a draft genome assembly of 1.47 Gbp for , of which more than half represent repeat elements. We predict around 29,000 protein-coding genes, with relatively large intron sizes, over 38,000 non-coding genes, and 105 miRNA loci. We further explore the high genetic variation (3% heterozygosity) within the species complex. Gene ontology reveals the most variable loci to be associated with pigmentation, development and immunity. The current work sets the stage for further development of genomic resources.
海洋环节动物是一种模式生物，用于包括进化与发育、神经生物学、生态学和再生等许多研究领域。在此，我们展示了（实验室培养参考基因组和单个个体组装基因组）以及与之密切相关的（单个个体组装基因组）的基因组，以促进比较基因组学方法的应用，并有助于探索生物学特性。我们使用长读长测序技术和染色体构象捕获技术，结合广泛的转录组资源，获得并注释了约1.47 Gbp的基因组组装草图，其中一半以上为重复元件。我们预测约有29,000个蛋白质编码基因，内含子相对较大，超过38,000个非编码基因，以及105个miRNA基因座。我们进一步探索了该物种复合体中的高遗传变异（约3%的杂合度）。基因本体论揭示，变异最大的基因座与色素沉着、发育和免疫相关。目前的工作为该物种基因组资源的进一步开发奠定了基础。
