Li Li, Rana Akshay N, Li Esther M, Travis Myesa O, Bruchas Michael R
Departments of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA 98195, USA.
Center for Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA 98195, USA.
bioRxiv. 2024 Jun 22:2024.06.18.599619. doi: 10.1101/2024.06.18.599619.
Matching arousal level to the motor activity of an animal is important for efficiently allocating cognitive resources and metabolic supply in response to behavioral demands, but how the brain coordinates changes in arousal and wakefulness in response to motor activity remains an unclear phenomenon. We hypothesized that the locus coeruleus (LC), as the primary source of cortical norepinephrine (NE) and promoter of cortical and sympathetic arousal, is well-positioned to mediate movement-arousal coupling. Here, using a combination of physiological recordings, fiber photometry, optogenetics, and behavioral tracking, we show that the LC activation is tightly coupled to the return of organized movements during waking from an anesthetized state. Moreover, in an awake animal, movement initiations are coupled to LC activation, while movement arrests, to LC deactivation. We also report that LC activity covaries with the depth of anesthesia and that LC photoactivation leads to sympathetic activation, consistent with its role in mediating increased arousal. Together, these studies reveal a more nuanced, modulatory role that LC plays in coordinating movement and arousal.
使动物的觉醒水平与运动活动相匹配，对于根据行为需求有效分配认知资源和代谢供应至关重要，但大脑如何协调觉醒和清醒状态以响应运动活动，仍是一个尚不清楚的现象。我们推测，蓝斑（LC）作为皮质去甲肾上腺素（NE）的主要来源以及皮质和交感神经觉醒的促进者，处于介导运动-觉醒耦合的有利位置。在此，我们结合生理记录、纤维光度测定法、光遗传学和行为跟踪研究表明，从麻醉状态苏醒至清醒过程中，LC激活与有组织运动的恢复紧密耦合。此外，在清醒动物中，运动起始与LC激活相关联，而运动停止则与LC失活相关联。我们还报告称，LC活动与麻醉深度相关，且LC光激活会导致交感神经激活，这与其在介导觉醒增加中的作用一致。这些研究共同揭示了LC在协调运动和觉醒方面发挥的更为细微、具有调节作用的角色。
