Lojo M M, Grau O
Mutat Res. 1985 Aug;143(4):201-5. doi: 10.1016/0165-7992(85)90081-8.
The assay of mutagenic activity of toxic drugs is difficult to perform and analyze, because one needs to know the kinetics of both effects in order to draw reliable conclusions. This is the case with niflumic acid (NA), which reduced the viability of S. typhimurium TA1535 100 times in the Ames test, but the background microcolonies show no difference from controls and the number of revertants was not altered by the drug. A test which measures the kinetics of growth of viable bacteria and mutants in liquid medium has been developed and applied to NA. No mutagenic activity was detected and elimination of the toxicity from the medium is suggested.
有毒药物的诱变活性测定难以进行和分析，因为为了得出可靠的结论，需要了解两种效应的动力学。尼氟酸（NA）就是这种情况，在艾姆斯试验中，它使鼠伤寒沙门氏菌TA1535的活力降低了100倍，但背景微菌落与对照无差异，且回复突变体的数量未因该药物而改变。已开发出一种测量液体培养基中活菌和突变体生长动力学的试验，并将其应用于NA。未检测到诱变活性，提示可消除培养基中的毒性。
