Fumero S, Berruto G P, Meriggi J, Mondino A, Silvestri S, Barale R
Farmaco Sci. 1981 Oct;36(10):867-74.
This study concerns the relationship between spontaneous revertants and number of bacterial cells plated in the Ames test. These two parameters do not appear to bear a direct relationship: marked variations in cell input are attended by negligible variations in spontaneous revertants. However, a relationship, described by a quadratic equation that approximates a hyperbole, exists between the spontaneous revertants and the number of total cellular divisions permitted by the histidine concentration in the medium. Furthermore a direct linear relationship has been observed between cell input and diameter of the plate background microcolonies. The problem of the toxic effect in the Ames test performed with drugs or substances having high bactericidal activity is discussed.
本研究关注艾姆斯试验中自发回复突变体与所接种细菌细胞数量之间的关系。这两个参数似乎并无直接关联：细胞接种量有显著变化时，自发回复突变体的变化却可忽略不计。然而，自发回复突变体与培养基中组氨酸浓度所允许的总细胞分裂数之间存在一种由近似双曲线的二次方程描述的关系。此外，已观察到细胞接种量与平板背景微菌落直径之间存在直接的线性关系。文中还讨论了在对具有高杀菌活性的药物或物质进行艾姆斯试验时的毒性作用问题。
