在母猪模型中，益生菌鼠李糖乳杆菌GG通过调节肠道微生物群和血清代谢物来改善胰岛素敏感性和后代存活率。

Probiotic Lactobacillus rhamnosus GG improves insulin sensitivity and offspring survival via modulation of gut microbiota and serum metabolite in a sow model.

作者信息

Gao Tianle, Li Ran, Hu Liang, Hu Quanfang, Wen Hongmei, Zhou Rui, Yuan Peiqiang, Zhang Xiaoling, Huang Lingjie, Zhuo Yong, Xu Shengyu, Lin Yan, Feng Bin, Che Lianqiang, Wu De, Fang Zhengfeng

机构信息

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, China.

Key Laboratory of Agricultural Product Processing and Nutrition Health (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, College of Food Science, Sichuan Agricultural University, Ya' an, 625014, China.

出版信息

J Anim Sci Biotechnol. 2024 Jul 2;15(1):89. doi: 10.1186/s40104-024-01046-z.

DOI:10.1186/s40104-024-01046-z

PMID:38951898

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11218078/

Abstract

BACKGROUND

Sows commonly experience insulin resistance in late gestation and lactation, causing lower feed intake and milk production, which can lead to higher mortality rates in newborn piglets. The probiotic Lactobacillus rhamnosus GG (LGG) is known to improve insulin resistance. However, whether supplementing LGG can improve insulin sensitivity in sows and enhance lactation performance, particularly the early survival of offspring remains unclear. Hence, we explored the effects and mechanisms of supplementing LGG during late gestation and lactation on sow insulin sensitivity, lactation performance, and offspring survival. In total, 20 sows were randomly allocated to an LGG (n = 10) and control group (n = 10).

RESULTS

In sows, LGG supplementation significantly improved insulin sensitivity during late gestation and lactation, increased feed intake, milk production and colostrum lactose levels in early lactation, and enhanced newborn piglet survival. Moreover, LGG treatment significantly reshaped the gut microbiota in sows, notably increasing microbiota diversity and enriching the relative abundance of insulin sensitivity-associated probiotics such as Lactobacillus, Bifidobacterium, and Bacteroides. Serum metabolite and amino acid profiling in late-gestation sows also revealed decreased branched-chain amino acid and kynurenine serum levels following LGG supplementation. Further analyses highlighted a correlation between mitigated insulin resistance in late pregnancy and lactation by LGG and gut microbiota reshaping and changes in serum amino acid metabolism. Furthermore, maternal LGG enhanced immunity in newborn piglets, reduced inflammation, and facilitated the establishment of a gut microbiota.

CONCLUSIONS

We provide the first evidence that LGG mitigates insulin resistance in sows and enhances offspring survival by modulating the gut microbiota and amino acid metabolism.

摘要

背景

母猪在妊娠后期和哺乳期通常会出现胰岛素抵抗，导致采食量和产奶量降低，这可能会导致新生仔猪死亡率升高。已知益生菌鼠李糖乳杆菌GG（LGG）可改善胰岛素抵抗。然而，补充LGG是否能提高母猪的胰岛素敏感性并增强泌乳性能，特别是后代的早期存活率，仍不清楚。因此，我们探讨了在妊娠后期和哺乳期补充LGG对母猪胰岛素敏感性、泌乳性能和后代存活率的影响及机制。总共20头母猪被随机分为LGG组（n = 10）和对照组（n = 10）。

结果

在母猪中，补充LGG显著提高了妊娠后期和哺乳期的胰岛素敏感性，增加了采食量、产奶量以及泌乳早期初乳中的乳糖水平，并提高了新生仔猪的存活率。此外，LGG处理显著重塑了母猪的肠道微生物群，显著增加了微生物群多样性，并丰富了与胰岛素敏感性相关的益生菌如乳酸杆菌、双歧杆菌和拟杆菌的相对丰度。妊娠后期母猪的血清代谢物和氨基酸谱分析还显示，补充LGG后支链氨基酸和犬尿氨酸血清水平降低。进一步分析强调了LGG减轻妊娠后期和哺乳期胰岛素抵抗与肠道微生物群重塑及血清氨基酸代谢变化之间的相关性。此外，母体LGG增强了新生仔猪的免疫力，减轻了炎症，并促进了肠道微生物群的建立。