Li T, Fang W, Chen S, Yang J, Zhao Y, Zhao S, Li T, Yang L, Guo Y, Liu Y
Dali Bai Autonomous Prefecture Institute of Schistosomiasis Control, Dali, Yunnan 671000, China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Health Commission Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai 200025, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2024 Apr 29;36(3):299-303. doi: 10.16250/j.32.1374.2022245.
To evaluate the potential risk of transmission of angiostrongyliasis by common freshwater snails in Dali Bai Autonomous Prefecture, Yunnan Province, so as to provide insights into local surveillance of angiostrongyliasis.
Common freshwater snails were collected from Dali Bai Autonomous Prefecture, Yunnan Province from March to April, 2020, and identified and bred in laboratory. SD rats were infected with third-stage larvae of that were isolated from commercially available snails in Dali Bai Autonomous Prefecture, and freshwater snails were infected with the first-stage larvae of that were isolated from the feces of SD rats 39 days post-infection at room temperature. The developmental process and morphological characteristics of worms in hosts were observed, and the percentages of infections in different species of freshwater snails were calculated. Then, SD rats were infected with the third-stage larvae of that were isolated from -infected freshwater snails, and the larval development and reproduction was observed.
More than 3 000 freshwater snail samples were collected from farmlands, ditches and wetlands around Erhai Lake in Dali Bai Autonomous Prefecture, and , , , , , , , spp., spp. and spp. were morphologically identified. A total of 105 commercially available snails were tested for infections, and 2 snails were found to be infected with , in which the third-stage larvae of were isolated. Ten species of freshwater snails were artificially infected with the third-stage larvae of , and all 10 species of freshwater snails were found to be infected with , with the highest positive rate of infections in spp. (62.3%, 137/204), and the lowest in (35.5%, 11/31). After SD rats were infected with the third-stage larvae of isolated from different species of freshwater snails, mature adult worms of were yielded.
Multiple species of freshwater snails may serve as intermediate hosts of under laboratory conditions in Dali Bai Autonomous Prefecture of Yunnan Province. Further investigations on natural infection of in wild snails in Dali Bai Autonomous Prefecture seem justified.
评估云南省大理白族自治州常见淡水螺传播广州管圆线虫病的潜在风险，为当地广州管圆线虫病监测提供依据。
于2020年3月至4月从云南省大理白族自治州采集常见淡水螺，在实验室进行鉴定和饲养。将从大理白族自治州市售福寿螺中分离出的第三期幼虫感染SD大鼠，在室温下将从感染后39天的SD大鼠粪便中分离出的第一期幼虫感染淡水螺。观察宿主体内虫体的发育过程和形态特征，计算不同种类淡水螺的感染率。然后，将从感染广州管圆线虫的淡水螺中分离出的第三期幼虫感染SD大鼠，观察幼虫的发育和繁殖情况。
从大理白族自治州洱海周边农田、沟渠和湿地采集了3000多个淡水螺样本，经形态学鉴定为中国圆田螺、铜锈环棱螺、方形环棱螺、梨形环棱螺、长角涵螺、纹沼螺、中华沼螺、放逸短沟蜷、福寿螺、耳萝卜螺。共检测105个市售福寿螺的广州管圆线虫感染情况，发现2个福寿螺感染广州管圆线虫，并从中分离出第三期幼虫。用广州管圆线虫第三期幼虫人工感染10种淡水螺，所有10种淡水螺均被感染，其中福寿螺的感染阳性率最高（62.3%，137/2名04），放逸短沟蜷最低（35.5%，11/31）。用从不同种类淡水螺中分离出的广州管圆线虫第三期幼虫感染SD大鼠后，获得了成熟的成虫。
在云南省大理白族自治州的实验室条件下，多种淡水螺可能作为广州管圆线虫的中间宿主。对大理白族自治州野生螺类广州管圆线虫自然感染情况进行进一步调查似乎是合理的。
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