Meier J, Stocker K, Svendsen L G, Brogli M
Toxicon. 1985;23(3):393-7. doi: 10.1016/0041-0101(85)90023-6.
Proteinase activities have been determined photometrically in 25 different Crotalidae and Viperidae snake venoms by using five different chromogenic proteinase substrates. The activity profiles obtained by listing the identity numbers of substrates hydrolyzed by the venoms in a decreasing potency order remained quite stable within a given snake population. Submission of a venom to various physical and chemical treatments did not alter its activity profile. It is therefore concluded that this simple method of determining proteinase activities could be of help in snake venom characterization, as well as in studying the influence of metallic ions and inhibitors of snake venom proteinases.
通过使用五种不同的生色蛋白酶底物，以光度法测定了25种不同的蝰蛇科和眼镜蛇科蛇毒中的蛋白酶活性。通过将毒液水解的底物的识别号按效力递减顺序列出而获得的活性谱在给定的蛇种群中保持相当稳定。对毒液进行各种物理和化学处理不会改变其活性谱。因此得出结论，这种测定蛋白酶活性的简单方法可能有助于蛇毒的鉴定，以及研究金属离子和蛇毒蛋白酶抑制剂的影响。
