Zhou Jinzhu, Wu Wei, Wang Dandan, Wang Wei, Chang Xinjian, Li Yunchuan, Li Jizong, Fan Baochao, Zhou Junming, Guo Rongli, Zhu Xuejiao, Li Bin
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.
Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou, China.
Front Microbiol. 2024 Jun 19;15:1418959. doi: 10.3389/fmicb.2024.1418959. eCollection 2024.
In recent years, porcine diarrhea-associated viruses have caused significant economic losses globally. These viruses present similar clinical symptoms, such as watery diarrhea, dehydration, and vomiting. Co-infections with porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are common. For the rapid and on-site preliminary diagnosis on the pig farms, this study aimed to develop a colloidal gold immunochromatography assay (GICA) strip for the detection of PEDV and TGEV simultaneously. The GICA kit showed that there was no cross-reactivity with the other five common porcine viruses. With visual observation, the lower limits were approximately 104 TCID50/mL and 104 TCID50/mL for PEDV and TGEV, respectively. The GICA strip could be stored at 4°C or 25°C for 12 months without affecting its efficacy. To validate the GICA strip, 121 clinical samples were tested. The positive rates of PEDV and TGEV were 42.9 and 9.9%, respectively, and the co-infection rate of the two viruses was 5.8% based on the duplex GICA strip. Thus, the established GICA strip is a rapid, specific, and stable tool for on-site preliminary diagnosis of PEDV- and TGEV-associated diarrhea.
近年来，猪腹泻相关病毒在全球范围内造成了重大经济损失。这些病毒表现出相似的临床症状，如水样腹泻、脱水和呕吐。猪流行性腹泻病毒（PEDV）和传染性胃肠炎病毒（TGEV）的共同感染很常见。为了在猪场进行快速的现场初步诊断，本研究旨在开发一种用于同时检测PEDV和TGEV的胶体金免疫层析测定（GICA）试纸条。GICA试剂盒显示与其他五种常见猪病毒无交叉反应。通过目视观察，PEDV和TGEV的下限分别约为104 TCID50/mL和104 TCID50/mL。GICA试纸条可在4°C或25°C下保存12个月而不影响其效力。为验证GICA试纸条，对121份临床样本进行了检测。基于双相GICA试纸条，PEDV和TGEV的阳性率分别为42.9%和9.9%，两种病毒的共同感染率为5.8%。因此，所建立的GICA试纸条是一种用于现场初步诊断PEDV和TGEV相关腹泻快速、特异且稳定的工具。
