Zhou Bokun, Sheng Qi, Yao Xinzhuan, Li Tong, Lu Litang
Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Science Guizhou University Guiyang China.
College of Tea Science, Institute of Plant Health and Medicine Guizhou University Guiyang China.
Plant Direct. 2024 Jul 3;8(7):e618. doi: 10.1002/pld3.618. eCollection 2024 Jul.
Tea plant ( [.]) is one of the most important crops in China, and tea branch is an important agronomic trait that determines the yield of tea plant. In previous work focused on GWAS that detecting GWAS signals related to plant architecture through whole genome re-sequencing of ancient tea plants, a gene locus TEA 029928 significantly related to plant type was found. Sequence alignment results showed that this gene belonged to the F-box family. We named it . CsBRC-GFP fusion proteins were mainly localized in the plasma membrane. By comparing the phenotypes of transgenic tobacco and WT tobacco, it was found that the number of branches of transgenic tobacco was significantly higher than that of wild-type tobacco. Through RNA-seq analysis, it was found that affects the branching development of plants by regulating the expression of genes related to brassinosteroid synthesis pathway in plants. In addition, overexpression of in rice could increase tiller number, grain length and width, and 1,000-grain weight.
茶树（[.]）是中国最重要的作物之一，茶树枝条是决定茶树产量的重要农艺性状。在先前通过对古茶树进行全基因组重测序来检测与植株形态相关的全基因组关联研究（GWAS）信号的工作中，发现了一个与植株类型显著相关的基因座TEA 029928。序列比对结果表明该基因属于F-box家族。我们将其命名为[具体名称未给出]。CsBRC-GFP融合蛋白主要定位于质膜。通过比较转基因烟草和野生型烟草的表型，发现转基因烟草的枝条数量显著高于野生型烟草。通过RNA测序分析发现，[具体基因未给出]通过调控植物油菜素内酯合成途径相关基因的表达来影响植物的分枝发育。此外，[具体基因未给出]在水稻中的过表达可增加分蘖数、粒长和粒宽以及千粒重。
