Matuo Y, Nishi N, Muguruma Y, Yoshitake Y, Kurata N, Wada F
Biochem Biophys Res Commun. 1985 Jul 16;130(1):293-300. doi: 10.1016/0006-291x(85)90416-4.
Isolated nuclei of the rat prostates contain a unique androgen-dependent basic protein, "probasin". Despite that it was hardly detectable in the cytosol centrifugally prepared from the prostates, immunofluorescent histological analysis of whole tissues using monoclonal antibody, which was raised against probasin purified from the nuclei, revealed that probasin was abundantly localized in the lumen and acinal regions of the epithelium, but hardly in the nuclei. Previous extraction of secretory fluid from the prostates caused about 60% decrease in the probasin content of isolated nuclei. These suggest that probasin was originally a secretory component in the prostates, being redistributed from the secretory fluid and granule into nuclei during fractionation of subcellular components.
大鼠前列腺的分离细胞核含有一种独特的雄激素依赖性碱性蛋白，即“前列腺碱性蛋白”。尽管从前列腺离心制备的胞质溶胶中几乎检测不到它，但使用针对从细胞核中纯化的前列腺碱性蛋白产生的单克隆抗体对整个组织进行免疫荧光组织学分析发现，前列腺碱性蛋白大量定位于上皮的管腔和腺泡区域，而在细胞核中几乎没有。先前从前列腺中提取分泌液导致分离细胞核中前列腺碱性蛋白含量降低约60%。这些表明前列腺碱性蛋白原本是前列腺中的一种分泌成分，在亚细胞成分分级分离过程中从分泌液和颗粒重新分布到细胞核中。
