Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece; Biomic AUTh, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Thessaloniki, 57001, Greece; FoodOmicsGR Research Infrastructure, AUTh Node, Center for Interdisciplinary Research and Innovation (CIRI-AUTH), Thessaloniki, 57001, Greece.
School of Chemical Engineering, Aristotle University of Thessaloniki, 54636, Thessaloniki, Greece.
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Aug 1;1243:124204. doi: 10.1016/j.jchromb.2024.124204. Epub 2024 Jun 15.
The production of biogenic amines (BAs), which are markers of both quality and safety in fish and fishery products, is influenced by the harvesting technique, handling, and other operations including those carried out on board the vessel. Scombroid dark-meat fish (e.g. tuna) are the fish species most frequently linked to histamine poisoning. The most commonly found BAs in fish are histamine, tyramine, putrescine, and cadaverine, which are produced when microbes decarboxylate the corresponding free amino acids. In this study, a rapid and cost-effective HILIC-MS/MS method was developed and validated for the determination of putrescine, cadaverine, histamine and tyramine in tuna samples. A simple sample preparation procedure was followed using the solvent mixture MeOH/HO (50/50, v/v), 0.1 % acetic acid for protein precipitation and analyte extraction. Intra- and inter-day accuracy, expressed as %Recovery (%R), ranged from 88.0 % (Cad) to 102.7 % (Tyr) and from 85.0 % (Cad) to 99.8 % (Tyr), respectively. Intra- and inter-day precision, expressed as %Relative Standard Deviation (%RSD), ranged from 0.4 % (Tyr, Put) to 3.3 % (His) and from 0.7 % (Tyr) to 5.0 % (Cad), respectively. Limits of detection (LOD) and quantification (LOQ) varied from 0.0009 to 0.0940 mg/kg and from 0.0030 mg/kg to 0.3100 mg/kg, respectively, depending on the analyte. Regarding the potential toxic effects linked to biogenic amines in foods, samples examined in this study showed no risk. The proposed method is an important analytical tool for routine analysis of BAs in fish products.
生物胺（BAs）的产生是鱼类和水产品质量和安全的标志，受到收获技术、处理以及包括船上操作在内的其他操作的影响。鲭鱼科暗色肉鱼类（例如金枪鱼）是与组胺中毒最相关的鱼类。鱼类中最常见的 BAs 是组胺、酪胺、腐胺和尸胺，它们是微生物脱羧相应游离氨基酸产生的。在这项研究中，开发并验证了一种快速且经济高效的亲水作用色谱-串联质谱（HILIC-MS/MS）方法，用于测定金枪鱼样品中的腐胺、尸胺、组胺和酪胺。采用甲醇/水（50/50，v/v）、0.1%乙酸的溶剂混合物进行简单的样品制备程序，用于蛋白质沉淀和分析物提取。日内和日间准确度，以回收率（%R）表示，范围为 88.0%（Cad）至 102.7%（Tyr）和 85.0%（Cad）至 99.8%（Tyr）。日内和日间精密度，以相对标准偏差（%RSD）表示，范围为 0.4%（Tyr、Put）至 3.3%（His）和 0.7%（Tyr）至 5.0%（Cad）。检测限（LOD）和定量限（LOQ）根据分析物的不同，范围为 0.0009 至 0.0940 mg/kg 和 0.0030 至 0.3100 mg/kg。考虑到食品中生物胺的潜在毒性影响，本研究中检查的样品没有风险。该方法是鱼类产品中 BAs 常规分析的重要分析工具。
