Key Laboratory of Global Changes and Biological Invasions, Bioscience and Technology College, Shenyang Agricultural University, Shenyang 110866, China.
Key Laboratory of Global Changes and Biological Invasions, Bioscience and Technology College, Shenyang Agricultural University, Shenyang 110866, China.
Protist. 2024 Aug;175(4):126047. doi: 10.1016/j.protis.2024.126047. Epub 2024 May 31.
Spirostomum is a genus of large ciliates, and its species are distributed worldwide. However, there has been limited research conducted on their geographical distribution and genomics. We obtained nine samples of ciliates from eight regions in Liaoning Province, China, and conducted a study on their geographical distribution and characteristics. Morphological and second-generation high-throughput sequencing methods were applied to identify the species, and a phylogenetic tree was established to gain a deeper understanding of the geographical distribution and evolutionary relationships of Spirostomum in Northeast China. The results identified Spirostomum yagiui and Spirostomum subtilis as a newly recorded species in Northeast China region. There are now five species of Spirostomum that have been recorded in China, and new details on the genomic characteristics of Spirostomum yagiui were provided. In addition, this study also identified the main branches of Spirostomum teres and Spirostomum minus in northern China, and provided a theoretical basis for the existence of hidden species. Spirostomum yagiui is the first species in the family Spirostomidae to have undergone mitochondrial genome sequencing.
旋口虫是一大类纤毛虫的属，其物种分布于世界各地。然而，对于它们的地理分布和基因组学的研究还很有限。我们从中国辽宁省的八个地区获得了九个纤毛虫样本，并对它们的地理分布和特征进行了研究。我们应用形态学和第二代高通量测序方法来鉴定物种，并建立了一个系统发育树，以更深入地了解旋口虫在东北地区的地理分布和进化关系。研究结果确定了日本旋口虫和细旋口虫为东北地区的新记录种。目前，中国已经记录了五种旋口虫，并且提供了日本旋口虫基因组特征的新细节。此外，本研究还确定了北方地区的扭口虫主要分支，为隐种的存在提供了理论依据。日本旋口虫是旋口科中第一个进行线粒体基因组测序的种。
