Geyman Logan J, Tanner Madeline P, Rosario-Meléndez Natalia, Peters Jason M, Mandel Mark J, van Kessel Julia C
Department of Biology, Indiana University , Bloomington, Indiana, USA.
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison , Madison, Wisconsin, USA.
Microbiol Resour Announc. 2024 Aug 13;13(8):e0004024. doi: 10.1128/mra.00040-24. Epub 2024 Jul 5.
Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn transposition. Here, we optimized methods specific for applying Mobile-CRISPRi in multiple species.
移动成簇规律间隔回文重复序列干扰（Mobile-CRISPRi）是一种已确立的用于细菌基因表达敲低的方法。失活的Cas9蛋白和向导RNA由异丙基β-D-1-硫代半乳糖苷诱导，并且所有组分通过Tn转座整合到染色体中。在此，我们优化了在多个物种中应用Mobile-CRISPRi的特定方法。
