缺乏指状结构域但具有高纤维蛋白亲和力的组织型纤溶酶原激活剂在大肠杆菌中的表达
Expression in E. coli of finger-domain lacking tissue-type plasminogen activator with high fibrin affinity.
作者信息
Kagitani H, Tagawa M, Hatanaka K, Ikari T, Saito A, Bando H, Okada K, Matsuo O
出版信息
FEBS Lett. 1985 Sep 9;189(1):145-9. doi: 10.1016/0014-5793(85)80860-7.
Tissue-type plasminogen activator (t-PA) has a high affinity for fibrin and induces lysis of fibrin (fibrinolysis) on the surface of fibrin without degrading circulating fibrinogen. cDNA for t-PA which lacks the 'finger-domain' (the site for fibrin affinity) was isolated from Detroit 562 cells. Analysis of the nucleotide sequence revealed a lack of the sequences which code for the finger-domain. A plasmid (pDPAT 1) containing the Escherichia coli tac promoter/operator and the cDNA sequence coding for 'finger-domain lacking t-PA' was constructed for expression in E. Coli. The polypeptide so produced was a new type of t-PA lacking finger-domain, but revealed plasminogen activator activity with the function of fibrin affinity.
组织型纤溶酶原激活剂（t-PA）对纤维蛋白具有高亲和力，并能在纤维蛋白表面诱导纤维蛋白溶解（纤溶作用），而不会降解循环中的纤维蛋白原。从底特律562细胞中分离出了缺乏“指状结构域”（纤维蛋白亲和力位点）的t-PA的cDNA。对核苷酸序列的分析显示，编码指状结构域的序列缺失。构建了一个含有大肠杆菌tac启动子/操纵子以及编码“缺乏指状结构域的t-PA”的cDNA序列的质粒（pDPAT 1），用于在大肠杆菌中表达。如此产生的多肽是一种新型的缺乏指状结构域的t-PA，但具有纤维蛋白亲和力功能的纤溶酶原激活剂活性。
Zotero 插件