Baldari C, Cesareni G
Gene. 1985;35(1-2):27-32. doi: 10.1016/0378-1119(85)90154-4.
We describe the construction and properties of pEMBLY plasmids. They belong to a new family of yeast shuttle vectors which are derived from plasmid vector pEMBL9 and offer the following improvement: relatively small size; large number of cloning sites; screening for insert-containing plasmids on indicator plates; different combinations of genes which complement auxotrophic deficiencies and sequences that support DNA replication in Saccharomyces cerevisiae; and ability to isolate the plasmid DNA in single-stranded (ss) form. The yeast S. cerevisiae can be efficiently transformed by these plasmids in both the ss and double-stranded (ds) forms. Finally, the presence of the phage f1 intergenic region allows one to obtain the cloned sequences in the ss form upon infection with the wild-type ss phage [Dotto et al., Virology 114 (1981) 463-473].
我们描述了pEMBLY质粒的构建及特性。它们属于酵母穿梭载体的一个新家族，这些载体源自质粒载体pEMBL9，并具有以下改进之处：相对较小的尺寸；大量的克隆位点；在指示平板上筛选含插入片段的质粒；互补营养缺陷型的不同基因组合以及支持酿酒酵母中DNA复制的序列；以及以单链（ss）形式分离质粒DNA的能力。酿酒酵母能够被这些单链和双链（ds）形式的质粒高效转化。最后，噬菌体f1基因间区域的存在使得在感染野生型单链噬菌体后能够获得单链形式的克隆序列[多托等人，《病毒学》114（1981）463 - 473] 。
