School of Life Sciences, Nantong University, 9 Seyuan Road, Nantong, Jiangsu, 226019, China; Analysis and Testing Center, Nantong University, 9 Seyuan Road, Nantong, Jiangsu, 226019, China.
School of Life Sciences, Nantong University, 9 Seyuan Road, Nantong, Jiangsu, 226019, China.
Anal Chim Acta. 2024 Aug 8;1316:342875. doi: 10.1016/j.aca.2024.342875. Epub 2024 Jun 15.
Indole-3-acetic acid (IAA) and salicylic acid (SA), pivotal regulators in plant growth, are integral to a variety of plant physiological activities. The ongoing and simultaneous monitoring of these hormones in vivo enhances our comprehension of their interactive and regulatory roles. Traditional detection methods, such as liquid chromatography-mass spectrometry, cannot obtain precise and immediate information on IAA and SA due to the complexity of sample processing. In contrast, the electrochemical detection method offers high sensitivity, rapid response times, and compactness, making it well-suited for in vivo or real-time detection applications.
A microneedle electrochemical sensor system crafted from disposable stainless steel (SS) wire was specifically designed for the real-time assessment of IAA and SA in plant in situ. This sensor system included a SS wire (100 μm diameter) coated with carbon cement and multi-walled carbon nanotubes, a plain platinum wire (100 μm diameter), and an Ag/AgCl wire (100 μm diameter). Differential pulse voltammetry and amperometry were adopted for detecting SA and IAA within the range of 0.1-20 μM, respectively. This sensor was applied to track IAA and SA fluctuations in tomato leaves during PstDC3000 infection, offering continuous data. Observations indicated an uptick in SA levels following infection, while IAA production was suppressed. The newly developed disposable SS wire-based microneedle electrochemical sensor system is economical, suitable for mass production, and inflicts minimal damage during the monitoring of SA and IAA in plant tissues.
This disposable microneedle electrochemical sensor facilitates in vivo detection of IAA and SA in smaller plant tissues and allows for long-time monitoring of their concentrations, which not only propels research into the regulatory and interaction mechanisms of IAA and SA but also furnishes essential tools for advancing precision agriculture.
吲哚-3-乙酸（IAA）和水杨酸（SA）作为植物生长的关键调节剂，是各种植物生理活动不可或缺的组成部分。在体内持续且同时监测这些激素，可以增进我们对它们相互作用和调节作用的理解。传统的检测方法，如液相色谱-质谱法，由于样品处理的复杂性，无法获得 IAA 和 SA 的精确和即时信息。相比之下，电化学检测方法具有高灵敏度、快速响应时间和紧凑性，非常适合体内或实时检测应用。
我们专门设计了一种由一次性不锈钢（SS）丝制成的微针电化学传感器系统，用于实时评估植物原位的 IAA 和 SA。该传感器系统包括一根涂有碳水泥和多壁碳纳米管的 SS 丝（100μm 直径）、一根普通铂丝（100μm 直径）和一根 Ag/AgCl 丝（100μm 直径）。差分脉冲伏安法和安培法分别用于检测 SA 和 IAA，检测范围分别为 0.1-20μM。该传感器被用于跟踪 PstDC3000 感染后番茄叶片中 IAA 和 SA 的波动，提供连续的数据。观察表明，感染后 SA 水平上升，而 IAA 产生受到抑制。新开发的基于一次性 SS 丝的微针电化学传感器系统经济实惠，适合大规模生产，并且在监测植物组织中的 SA 和 IAA 时造成的损伤最小。
这种一次性微针电化学传感器能够在较小的植物组织中进行体内检测 IAA 和 SA，并允许长时间监测它们的浓度，这不仅推动了对 IAA 和 SA 调节和相互作用机制的研究，还为推进精准农业提供了必要的工具。
