Center for Genomics, Helmy Institute for Medical Sciences, Zewail City of Science and Technology, Giza, Egypt.
Biomedical Sciences Program, University of Science and Technology, Zewail City of Science and Technology, Giza, Egypt.
Sci Rep. 2024 Jul 5;14(1):15500. doi: 10.1038/s41598-024-65178-y.
The incidence of Pseudomonas aeruginosa infections in healthcare environments, particularly in low-and middle-income countries, is on the rise. The purpose of this study was to provide comprehensive genomic insights into thirteen P. aeruginosa isolates obtained from Egyptian healthcare settings. Phenotypic analysis of the antimicrobial resistance profile and biofilm formation were performed using minimum inhibitory concentration and microtiter plate assay, respectively. Whole genome sequencing was employed to identify sequence typing, resistome, virulome, and mobile genetic elements. Our findings indicate that 92.3% of the isolates were classified as extensively drug-resistant, with 53.85% of these demonstrating strong biofilm production capabilities. The predominant clone observed in the study was ST773, followed by ST235, both of which were associated with the O11 serotype. Core genome multi-locus sequence typing comparison of these clones with global isolates suggested their potential global expansion and adaptation. A significant portion of the isolates harbored Col plasmids and various MGEs, all of which were linked to antimicrobial resistance genes. Single nucleotide polymorphisms in different genes were associated with the development of antimicrobial resistance in these isolates. In conclusion, this pilot study underscores the prevalence of extensively drug-resistant P. aeruginosa isolates and emphasizes the role of horizontal gene transfer facilitated by a diverse array of mobile genetic elements within various clones. Furthermore, specific insertion sequences and mutations were found to be associated with antibiotic resistance.
在医疗环境中，特别是在中低收入国家，铜绿假单胞菌感染的发病率正在上升。本研究的目的是提供对从埃及医疗环境中获得的十三株铜绿假单胞菌分离株的综合基因组见解。使用最低抑菌浓度和微量滴定板测定法分别对药敏表型分析和生物膜形成进行了分析。全基因组测序用于鉴定序列分型、耐药组、毒力组和可移动遗传元件。我们的研究结果表明，92.3%的分离株被归类为广泛耐药，其中 53.85%的分离株表现出强烈的生物膜形成能力。本研究中观察到的主要克隆是 ST773，其次是 ST235，它们都与 O11 血清型有关。对这些克隆与全球分离株进行的核心基因组多位点序列分型比较表明，它们具有潜在的全球扩张和适应能力。相当一部分分离株携带 Col 质粒和各种 MGEs，这些都与抗菌药物耐药基因有关。不同基因中的单核苷酸多态性与这些分离株中抗菌药物耐药性的发展有关。总之，这项初步研究强调了广泛耐药铜绿假单胞菌分离株的流行，并强调了各种克隆中多样化的可移动遗传元件促进水平基因转移的作用。此外，还发现特定的插入序列和突变与抗生素耐药性有关。
