An Jia, Hu Nan, Yin Chengyue, Liu Yufei
School of Optoelectronic Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.
Key Laboratory of Optoelectronic Technology & Systems (Chongqing University), Ministry of Education, Chongqing 400044, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 5;322:124744. doi: 10.1016/j.saa.2024.124744. Epub 2024 Jul 2.
In this work, to enhance the fluorescence quantum yield of carbon dots (CDs), a novel metal-enhanced fluorescence (MEF) structure was designed by decorating CDs on silver nanoparticle (AgNPs) film. The glass slide-AgNPs (GS-AgNPs) structure was fabricated using the electrostatic adsorption method, and the AgNPs-CDs structures were prepared by the direct drying method, which then formed the GS-AgNPs-CDs composite structure. In this structure, the MEF effect was found to be size dependent by changing the 5 types of AgNPs with different sizes. And the MEF effect also decreased as the distance between the AgNPs and CDs increased by using polyvinylpyrrolidone (PVP) to separate the AgNPs and CDs. This hybrid structure can be used as a fluorescence detection platform and the recorded fluorescence intensity of GS-AgNPs 428 nm-CDs achieved a maximum enhancement factor (EF) of 31.72. Considering the high enhancement factor, this system may become promising to find potential applications in biochemical assay fields.
在本工作中，为提高碳点（CDs）的荧光量子产率，通过在银纳米颗粒（AgNPs）薄膜上修饰CDs设计了一种新型的金属增强荧光（MEF）结构。采用静电吸附法制备了玻片-AgNPs（GS-AgNPs）结构，并通过直接干燥法制备了AgNPs-CDs结构，进而形成了GS-AgNPs-CDs复合结构。在该结构中，通过改变5种不同尺寸的AgNPs发现MEF效应与尺寸有关。并且，通过使用聚乙烯吡咯烷酮（PVP）来分离AgNPs和CDs，随着AgNPs与CDs之间距离的增加，MEF效应也会降低。这种混合结构可作为荧光检测平台，所记录的GS-AgNPs 428 nm-CDs的荧光强度实现了31.72的最大增强因子（EF）。考虑到高增强因子，该系统在生化分析领域寻找潜在应用方面可能具有前景。
