Liaoning Key Lab for Aquatic Processing Quality and Safety, Dalian Polytechnic University, Dalian 116034, Liaoning Province, China.
Department of Inorganic Nonmetallic Materials Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning Province, China.
Int J Biol Macromol. 2024 Sep;276(Pt 1):133689. doi: 10.1016/j.ijbiomac.2024.133689. Epub 2024 Jul 5.
Benzyl isothiocyanate (BITC) is a naturally active bacteriostatic substance and κ-carrageenan (KC) is a good film-forming substrate. In the present study, a nanoemulsion incorporating BITC was fabricated with a particle size of 224.1 nm and an encapsulation efficiency of 69.2 %. Subsequently, the acquired BITC nanoemulsion (BITC-NE) was incorporated into the KC-based film, and the light transmittance of the prepared composite films was lower than that of the pure KC film. Fourier transform infrared spectroscopy and scanning electron microscopy revealed that BITC-NE was compatible with the KC matrix. BITC-NE incorporation enhanced the tensile strength of the KC-based films by 33.7 %, decreased the elongation at break by 33.8 %, decreased the water vapor permeability by 60.1 %, increased the maximum thermal degradation temperature by 48.8 %, and decreased the oxygen permeability by 42 % (p < 0.05). Furthermore, the composite films showed enhanced antimicrobial activity against Staphylococcus aureus, Salmonella typhimurium, and Pseudomonas fluorescens. The developed KC-based composite films were applied to wrap raw beef, which significantly delayed the increase in total viable count, total volatile base nitrogen content, and thiobarbituric acid reactive substances, and prolonged the shelf-life of the raw beef by up to 10 days. These results indicated that the composite films prepared by incorporating BITC nanoemulsions into KC matrices have great antimicrobial application potential.
苄基异硫氰酸酯（BITC）是一种天然活性抑菌物质，κ-卡拉胶（KC）是一种良好的成膜基质。本研究制备了一种粒径为 224.1nm、包封率为 69.2%的 BITC 纳米乳液。随后，将所得的 BITC 纳米乳液（BITC-NE）掺入 KC 基膜中，所制备的复合膜的透光率低于纯 KC 膜。傅里叶变换红外光谱和扫描电子显微镜表明 BITC-NE 与 KC 基质相容。BITC-NE 的掺入使 KC 基膜的拉伸强度提高了 33.7%，断裂伸长率降低了 33.8%，水蒸气透过率降低了 60.1%，最大热降解温度提高了 48.8%，氧气透过率降低了 42%（p<0.05）。此外，复合膜对金黄色葡萄球菌、鼠伤寒沙门氏菌和荧光假单胞菌表现出增强的抗菌活性。所开发的基于 KC 的复合膜被用于包裹生牛肉，显著延缓了总活菌数、总挥发性碱基氮含量和硫代巴比妥酸反应物质的增加，并将生牛肉的保质期延长了长达 10 天。这些结果表明，将 BITC 纳米乳液掺入 KC 基质中制备的复合膜具有很大的抗菌应用潜力。
