Wu Hongyan, Ao Xinying, Liu Jianan, Zhu Junya, Bi Jingran, Hou Hongman, Hao Hongshun, Zhang Gongliang
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Liaoning Key Lab for Aquatic Processing Quality and Safety, Dalian 116034, China.
Foods. 2022 Sep 2;11(17):2687. doi: 10.3390/foods11172687.
A bioactive packaging material based on chitosan (CS) incorporated with benzyl isothiocyanate (BITC) and α-cyclodextrin (α-CD) was fabricated to evaluate its preservative effects on fresh beef stored at 4 °C for 12 d according to the quality analysis. The Fourier-transform infrared (FTIR) spectrum revealed that the major structural moiety of BITC was embedded in the cavity of α-CD, except for the thiocyanate group. FTIR and X-ray diffraction analysis further verified that intermolecular interactions were formed between the BITC-α-CD and CS film matrix. The addition of BITC-α-CD decreased the UV light transmittance of pure CS film to lower than 63% but still had enough transparency for observing packaged items. The CS-based composite film displayed a sustainable antibacterial capacity and an enhanced antioxidant activity. Moreover, the total viable counts, total volatile base nitrogen, pH, thiobarbituric acid-reactive substances, and sensory evaluation of the raw beef treated with the CS-based composite film were 6.31 log colony-forming unit (CFU)/g, 19.60 mg/100 g, 6.84, 0.26 mg/kg, and 6.5 at 12 days, respectively, indicating the favorable protective efficacy on beef. These results suggested that the fabricated CS-based composite film has the application potential to be developed as a bioactive food packaging material, especially for beef preservation.
制备了一种基于壳聚糖（CS）并掺入异硫氰酸苄酯（BITC）和α-环糊精（α-CD）的生物活性包装材料，根据质量分析评估其对4℃储存12天的新鲜牛肉的保鲜效果。傅里叶变换红外（FTIR）光谱显示，除硫氰酸酯基团外，BITC的主要结构部分嵌入α-CD的空腔中。FTIR和X射线衍射分析进一步证实，BITC-α-CD与CS薄膜基质之间形成了分子间相互作用。BITC-α-CD的加入使纯CS薄膜的紫外光透过率降低至63%以下，但仍具有足够的透明度以观察包装物品。基于CS的复合薄膜表现出可持续的抗菌能力和增强的抗氧化活性。此外，用基于CS的复合薄膜处理的生牛肉在12天时的总活菌数、总挥发性盐基氮、pH值、硫代巴比妥酸反应物质和感官评价分别为6.31 log菌落形成单位（CFU）/g、19.60 mg/100 g、6.84、0.26 mg/kg和6.5，表明对牛肉具有良好的保护效果。这些结果表明，制备的基于CS的复合薄膜具有作为生物活性食品包装材料开发的应用潜力，特别是用于牛肉保鲜。
