College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China.
Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China.
Gene. 2024 Nov 15;927:148751. doi: 10.1016/j.gene.2024.148751. Epub 2024 Jul 4.
By analyzing the expression patterns of inner root sheath (IRS) specific genes during different developmental stages of hair follicle (HF) in Tan sheep embryos and at birth, this study aims to reveal the influence of the IRS on crimped wool. Skin tissues from the scapular region of male Tan sheep were collected at 85 days (E85) and 120 days (E120) of fetal development, and at 0 days (D0), 35 days (D35), and 60 days (D60) after birth, with four samples at each stage. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to determine the relative expression levels of IRS type I keratin genes (KRT25, KRT26, KRT27, KRT28), type II keratin genes (KRT71, KRT72, KRT73, KRT74), and the trichohyalin gene (TCHH) in the skin of Tan sheep at different stages. Results showed that the expression levels of all IRS-specific genes peaked at D0, with the expression of all genes significantly higher than at E85 (P < 0.01), except for KRT73 and TCHH. The expression levels of KRT25, KRT26, and KRT72 were also significantly higher than at E120 (P < 0.01). Furthermore, the expression levels of KRT27, KRT28, KRT71, and KRT74 were significantly higher than both at E120 and D35 (P < 0.01). The expression levels of other genes at different stages showed no significant difference (P > 0.05). Conclusion: The IRS-specific genes exhibit the highest expression levels in Tan sheep at the neonatal stage. The expression levels of KRT71, KRT72, and TCHH, which are consistent with the pattern of wool crimp, may influence the morphology of the IRS and thereby affect the crimp of Tan sheep wool.
本研究通过分析滩羊胚胎和出生时毛囊不同发育阶段内根鞘（IRS）特异性基因的表达模式，旨在揭示 IRS 对卷曲羊毛的影响。从雄性滩羊的肩胛区采集 85 天（E85）和 120 天（E120）胎儿发育以及 0 天（D0）、35 天（D35）和 60 天（D60）出生后的皮肤组织，每个阶段采集 4 个样本。采用实时定量聚合酶链反应（RT-qPCR）测定 IRS Ⅰ型角蛋白基因（KRT25、KRT26、KRT27、KRT28）、Ⅱ型角蛋白基因（KRT71、KRT72、KRT73、KRT74）和 trichohyalin 基因（TCHH）在滩羊皮肤不同阶段的相对表达水平。结果表明，所有 IRS 特异性基因的表达水平在 D0 时达到峰值，所有基因的表达均显著高于 E85（P<0.01），除了 KRT73 和 TCHH。KRT25、KRT26 和 KRT72 的表达水平也显著高于 E120（P<0.01）。此外，KRT27、KRT28、KRT71 和 KRT74 的表达水平也显著高于 E120 和 D35（P<0.01）。其他基因在不同阶段的表达水平无显著差异（P>0.05）。结论：IRS 特异性基因在滩羊新生阶段表达水平最高。KRT71、KRT72 和 TCHH 的表达模式与羊毛卷曲一致，可能影响 IRS 的形态，从而影响滩羊羊毛的卷曲。
