Shi An, Ma Sijia, Yang Zhuo, Ding Wei, Tian Jinyang, Chen Xin, Tao Jinzhong
College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China.
Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China.
Animals (Basel). 2024 Oct 4;14(19):2858. doi: 10.3390/ani14192858.
Crimped wool in Tan sheep gradually transitions to straight wool after 35 days (the er-mao stage), which reduces its commercial value. To investigate the changes in wool proteins during this stage, we performed comparative proteomic analysis of the straight and crimped wool using tandem mass tag (TMT)-based quantification. The mean fur curvature (MFC) of crimped wool was significantly greater than that of straight wool ( < 0.001). We identified 1218 proteins between the two types of wool, including 50 keratins (Ks) and 10 keratin-associated proteins (KAPs). There were 213 differentially expressed proteins, including 13 Ks and 4 KAPs. Crimped wool showed relatively high abundances of KAP24-1, K84, K32, K82, and intermediate filament rod domain-containing protein (IRDC), whereas straight wool had relatively high abundances of K6A, K27, K80, KAP16-1, KAP27-1, and trichohyalin (TCHH). The expression levels of KAP16-1, KAP24-1, and KAP27-1 were related to the ratio of paracortex, which may be associated with wool crimp formation. Additionally, high expressions of TCHH, K27, and K6A in the inner root sheath (IRS) were linked to fiber fineness in straight wool. These findings provide insight into the overall expression and distribution patterns of Ks and KAPs, offering opportunities to improve wool quality and enhance its economic potential in the textile industry.
滩羊的卷曲毛在35天（二毛期）后逐渐转变为直毛，这降低了其商业价值。为了研究此阶段羊毛蛋白质的变化，我们使用基于串联质谱标签（TMT）定量的方法对直毛和卷曲毛进行了比较蛋白质组学分析。卷曲毛的平均毛曲率（MFC）显著大于直毛（<0.001）。我们在两种类型的羊毛中鉴定出1218种蛋白质，包括50种角蛋白（Ks）和10种角蛋白相关蛋白（KAPs）。有213种差异表达蛋白，包括13种Ks和4种KAPs。卷曲毛中KAP24-1、K84、K32、K82和含中间丝杆状结构域蛋白（IRDC）的丰度相对较高，而直毛中K6A、K27、K80、KAP16-1、KAP27-1和毛透明蛋白（TCHH）的丰度相对较高。KAP16-1、KAP24-1和KAP27-1的表达水平与副皮质比例有关，这可能与羊毛卷曲的形成有关。此外，内根鞘（IRS）中TCHH、K27和K6A的高表达与直毛的纤维细度有关。这些发现为Ks和KAPs的整体表达和分布模式提供了见解，为改善羊毛质量和提高其在纺织工业中的经济潜力提供了机会。
