Department of Molecular Physiology and Biological Physics, Center for Membrane and Cell Physiology, University of Virginia, Charlottesville, VA, United States.
Institut Curie, Université PSL, Sorbonne Université, CNRS UMR168, Physics of Cells and Cancer, Paris, France.
Methods Enzymol. 2024;700:275-294. doi: 10.1016/bs.mie.2024.04.024.
Synthetic model membranes are important tools to elucidate lipid domain and protein interactions due to predefined lipid compositions and characterizable biophysical properties. Here, we introduce a model membrane with multiple lipid bilayers (multi-bilayers) stacked on a mica substrate that is prepared through a spin-coating technique. The spin-coated multi-bilayers are useful in the study of phase separated membranes with a high cholesterol content, mobile lipids, microscopic and reversible phase separation, and easy conjugation with proteins, which make them a good model to study interactions between proteins and membrane domains.
人工合成的模型膜是研究脂质域和蛋白质相互作用的重要工具，因为它们具有预定的脂质组成和可描述的物理特性。在这里，我们介绍了一种通过旋涂技术在云母基底上制备的具有多层脂质双层（多双层）的模型膜。该旋涂多层膜适用于研究具有高胆固醇含量、可移动脂质、微观和可逆相分离的相分离膜，并且易于与蛋白质偶联，因此它们是研究蛋白质与膜域相互作用的良好模型。
