Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) / Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL) / Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
J Reprod Immunol. 2024 Sep;165:104289. doi: 10.1016/j.jri.2024.104289. Epub 2024 Jun 29.
One of the initial causes of cystic ovarian disease (COD) is a failure in the normal ovulation mechanism. This study aimed to characterize the populations of immune cells (T-lymphocytes, B-lymphocytes, monocytes-macrophages and granulocytes) present in the ovary of cows with COD and induced follicular persistence, and evaluate their relation with follicular persistence and cyst formation. The follicular persistence model was developed using a progesterone (P4) slow-release intravaginal device, to obtain subluteal concentrations of P4. Results evidenced that T-lymphocytes, B-lymphocytes and monocytes-macrophages in the cortex, medulla, and theca externa and interna of dominant follicles were higher in the control group than in the COD and all persistence groups. Granulocytes in the medulla and theca externa of dominant follicles were lower in the control group than in the COD group, and those in the cortex and medulla were lower in the control group than in the persistence groups. The presence of T-lymphocytes, B-lymphocytes and granulocytes in the follicular fluid was abundant, especially that of granulocytes, without differences between control and COD cows. These results suggest that the immune system potentially plays a role in the local mechanisms of COD pathogenesis in dairy cows. In spontaneous COD and in our follicular persistence model, the distribution of the cells studied was different from that in the control group. However, to our knowledge, this is the first report describing the presence of immune cells in bovine follicular fluid samples and the expression of steroid hormone receptors in infiltrating immune cells in the bovine ovary.
多囊卵巢疾病（COD）的最初原因之一是正常排卵机制的失败。本研究旨在描述患有 COD 和诱导卵泡持续存在的奶牛卵巢中存在的免疫细胞（T 淋巴细胞、B 淋巴细胞、单核细胞-巨噬细胞和粒细胞）群体，并评估它们与卵泡持续存在和囊肿形成的关系。使用孕激素（P4）缓释阴道装置开发卵泡持续存在模型，以获得 P4 的亚黄体浓度。结果表明，在优势卵泡的皮质、髓质和外膜和内膜中，T 淋巴细胞、B 淋巴细胞和单核细胞-巨噬细胞在对照组中高于 COD 和所有持续存在组。在优势卵泡的髓质和外膜中，粒细胞在对照组中低于 COD 组，在对照组中低于持续存在组。卵泡液中 T 淋巴细胞、B 淋巴细胞和粒细胞的存在丰富，尤其是粒细胞，对照组和 COD 奶牛之间没有差异。这些结果表明免疫系统可能在奶牛 COD 发病机制的局部机制中发挥作用。在自发性 COD 和我们的卵泡持续存在模型中，研究中细胞的分布与对照组不同。然而，据我们所知，这是首次描述在牛卵泡液样本中存在免疫细胞以及浸润免疫细胞中类固醇激素受体的表达。
