Stassi Antonela F, Gareis Natalia C, Marelli Belkis E, Matiller Valentina, Leiva Cristian J M, Rey Florencia, Ortega Hugo H, Salvetti Natalia R, Baravalle M Eugenia
Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas, (CONICET) -Universidad Nacional del Litoral, Argentina.
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina.
Zygote. 2019 Oct;27(5):285-298. doi: 10.1017/S0967199419000285. Epub 2019 Aug 15.
Ovulation is considered an inflammatory, cytokine-mediated event. Cytokines, which are recognized as growth factors with immunoregulatory properties, are involved in many cellular processes at the ovarian level. In this sense, cytokines affect fertility and are involved in the development of different ovarian disorders such as bovine cystic ovarian disease (COD). Because it has been previously demonstrated that ovarian cells represent both sources and targets of cytokines, the aim of this study was to examine the expression of several cytokines, including IL-1β, IL-1RA, IL-1RI, IL-1RII, IL-4 and IL-8, in ovarian follicular structures from cows with spontaneous COD. The protein expression of these cytokines was evaluated by immunohistochemistry. Additionally, IL-1β, IL-4 and IL-8 concentrations in follicular fluid (FF) and serum were determined by enzyme-linked immunosorbent assay (ELISA). In granulosa and theca cells, IL-1RI, IL-1RII, IL-1RA and IL-4 expression levels were higher in cystic follicles than in the control dominant follicles. The serum and FF concentrations of IL-1β and IL-4 showed no differences between groups, whereas IL-8 concentration was detected only in FF of cysts from cows with COD. The FF and serum concentrations of IL-1β and IL-8 showed no significant differences, whereas IL-4 concentration was higher in FF than in serum in both the control and COD groups. These results evidenced an altered expression of cytokines in ovaries of cows with COD that could contribute to the pathogenesis of this disease.
排卵被认为是一个炎症性的、由细胞因子介导的过程。细胞因子被认为是具有免疫调节特性的生长因子，参与卵巢水平的许多细胞过程。从这个意义上讲，细胞因子影响生育能力，并参与不同卵巢疾病的发展，如牛的囊性卵巢疾病（COD）。由于先前已经证明卵巢细胞既是细胞因子的来源又是其靶标，因此本研究的目的是检测几种细胞因子（包括IL-1β、IL-1RA、IL-1RI、IL-1RII、IL-4和IL-8）在患有自发性COD的奶牛卵巢卵泡结构中的表达。通过免疫组织化学评估这些细胞因子的蛋白表达。此外，通过酶联免疫吸附测定（ELISA）测定卵泡液（FF）和血清中IL-1β、IL-4和IL-8的浓度。在颗粒细胞和卵泡膜细胞中，囊性卵泡中IL-1RI、IL-1RII、IL-1RA和IL-4的表达水平高于对照优势卵泡。IL-1β和IL-4的血清和FF浓度在各组之间没有差异，而仅在患有COD的奶牛囊肿的FF中检测到IL-8浓度。IL-1β和IL-8的FF和血清浓度没有显著差异，而在对照组和COD组中，FF中的IL-4浓度均高于血清。这些结果表明，患有COD的奶牛卵巢中细胞因子表达发生改变，这可能有助于该疾病的发病机制。
