Ashcroft S J, Lowry M
Diabetologia. 1979 Sep;17(3):165-8. doi: 10.1007/BF01219744.
The ability of all eight D-aldohexose steroisomers to stimulate insulin release and biosynthesis was compared with their ability to serve as a metabolic substrate for isolated islets of Langerhans as judged by formation of lactate. Insulin release and synthesis were stimulated by glucose or mannose but not by allose, altrose, gulose, idose, galactose or talose. No potentiary effects of allose, altrose, gulose, idose, or talose were found on insulin release in the presence of 4 mmol/l glucose nor did these sugars inhibit insulin release in the presence of 20 mmol/l glucose. Lactate formation was increased above values found in the absence of added substrate by 20 mmol/l D-glucose or mannose, but not by allose, altrose, gulose, galactose or talose. The results support the substrate-site hypothesis for the recognition of sugars as stimuli of insulin release and synthesis.
通过乳酸的生成来判断，将所有八种D-醛己糖立体异构体刺激胰岛素释放和生物合成的能力与其作为分离的胰岛朗格汉斯细胞代谢底物的能力进行了比较。葡萄糖或甘露糖可刺激胰岛素释放和合成，但阿洛糖、阿卓糖、古洛糖、艾杜糖、半乳糖或塔罗糖则无此作用。在4 mmol/l葡萄糖存在的情况下，未发现阿洛糖、阿卓糖、古洛糖、艾杜糖或塔罗糖对胰岛素释放有增强作用，在20 mmol/l葡萄糖存在的情况下，这些糖也未抑制胰岛素释放。20 mmol/l D-葡萄糖或甘露糖可使乳酸生成量高于未添加底物时的值，但阿洛糖、阿卓糖、古洛糖、半乳糖或塔罗糖则无此作用。这些结果支持了糖作为胰岛素释放和合成刺激物识别的底物位点假说。
