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TNNT1 可加速肺癌细胞的迁移、侵袭和 EMT 进程。

TNNT1 accelerates migration, invasion and EMT progression in lung cancer cells.

机构信息

Department of Acupuncture-Moxibustion and Tuina, Qilu Hospital of Shandong University, Jinan, China.

Ankang Hospital of Jinan, Jinan, China.

出版信息

Thorac Cancer. 2024 Aug;15(23):1749-1756. doi: 10.1111/1759-7714.15400. Epub 2024 Jul 7.

DOI:10.1111/1759-7714.15400
PMID:38973201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11320084/
Abstract

BACKGROUND

Clinically, most patients with lung cancer (LC) die from tumor spread and metastasis. Specific metastasis-related molecules can provide reference for clinical prediction of efficacy, evaluation of prognosis, and search for the best treatment plan. Troponin T1 (TNNT1) is highly expressed in various cancer tissues, which affects malignant behavior of tumor cells and is related to patients' survival and prognosis. However, the role and molecular mechanism of TNNT1 in LC invasion and metastasis have not yet been investigated.

METHODS

Gene expression profiling interactive analysis (GEPIA) online analysis was used to analyze TNNT1 expression in LC tissues. Quantitative real-time-polymerase chain reaction (qRT-PCR) or western blot were performed to measure TNNT1 or epithelial-to-mesenchymal transition (EMT)-related and Wnt/β-catenin pathway-related protein expression in LC cells. After TNNT1 knockdown, cell scratch healing and transwell assays were introduced to assess cell migration and invasion, respectively.

RESULTS

TNNT1 expression in LC tissues and cells was increased. TNNT1 knockdown notably impaired LC cell migration, invasion and EMT. TNNT1 knockdown inhibited Wnt/β-catenin pathway of LC cells. Lithium chloride (LiCl) addition partially restored the inhibition of TNNT1 knockdown on migration, invasion, EMT and Wnt/β-catenin of LC cells.

CONCLUSION

TNNT1 knockdown attenuated LC migration, invasion and EMT, possibly through Wnt/β-catenin signaling.

摘要

背景

临床上，大多数肺癌 (LC) 患者死于肿瘤扩散和转移。特定的转移相关分子可为临床疗效预测、预后评估和寻找最佳治疗方案提供参考。肌钙蛋白 T1（TNNT1）在各种癌组织中高表达，影响肿瘤细胞的恶性行为，与患者的生存和预后有关。然而，TNNT1 在 LC 侵袭和转移中的作用及其分子机制尚未得到研究。

方法

采用基因表达谱交互分析（GEPIA）在线分析方法分析 LC 组织中 TNNT1 的表达。采用定量实时聚合酶链反应（qRT-PCR）或 Western blot 检测 LC 细胞中 TNNT1 或上皮间质转化（EMT）相关和 Wnt/β-连环蛋白通路相关蛋白的表达。在 TNNT1 敲低后，引入细胞划痕愈合和 Transwell 测定分别评估细胞迁移和侵袭。

结果

LC 组织和细胞中的 TNNT1 表达增加。TNNT1 敲低显著损害 LC 细胞的迁移、侵袭和 EMT。TNNT1 敲低抑制 LC 细胞的 Wnt/β-连环蛋白通路。氯化锂 (LiCl) 加药部分恢复了 TNNT1 敲低对 LC 细胞迁移、侵袭、EMT 和 Wnt/β-连环蛋白的抑制作用。

结论

TNNT1 敲低可减弱 LC 的迁移、侵袭和 EMT，可能通过 Wnt/β-连环蛋白信号通路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/b9e9e0daeedb/TCA-15-1749-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/930887d0497b/TCA-15-1749-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/6d003b5f6168/TCA-15-1749-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/1dfc1b94f416/TCA-15-1749-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/2a54648747b4/TCA-15-1749-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/b9e9e0daeedb/TCA-15-1749-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/930887d0497b/TCA-15-1749-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/6d003b5f6168/TCA-15-1749-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/1dfc1b94f416/TCA-15-1749-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/2a54648747b4/TCA-15-1749-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e96d/11320084/b9e9e0daeedb/TCA-15-1749-g002.jpg

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J Endocrinol Invest. 2024 Jan;47(1):149-166. doi: 10.1007/s40618-023-02132-1. Epub 2023 Jul 21.
4
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Gene. 2023 May 20;865:147331. doi: 10.1016/j.gene.2023.147331. Epub 2023 Mar 5.
5
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Iran J Biotechnol. 2023 Jan 1;21(1):e3405. doi: 10.30498/ijb.2022.344921.3405. eCollection 2023 Jan.
6
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7
Emerging roles of intratumor microbiota in cancer metastasis.肿瘤内微生物组在癌症转移中的新兴作用。
Trends Cell Biol. 2023 Jul;33(7):583-593. doi: 10.1016/j.tcb.2022.11.007. Epub 2022 Dec 14.
8
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Semin Cancer Biol. 2022 Dec;87:17-31. doi: 10.1016/j.semcancer.2022.10.006. Epub 2022 Oct 27.
9
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J Hematol Oncol. 2022 Sep 8;15(1):129. doi: 10.1186/s13045-022-01347-8.
10
Lung Cancer Treatment Advances in 2022.2022 年肺癌治疗进展。
Cancer Invest. 2023 Jan;41(1):12-24. doi: 10.1080/07357907.2022.2119479. Epub 2022 Dec 12.