Amjad Fatemeh, Keshvari Hamid, Dalman Azam, Montazeri Leila
Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Int J Fertil Steril. 2024 Jun 9;18(3):286-292. doi: 10.22074/ijfs.2023.1990652.1443.
An artificial ovary based on the alginate (ALG) hydrogel has been widely implemented to preserve prepubertal female fertility. However, this platform is not fully capable of successful an ovary microenvironment simulation for follicle development, holding great potential for its improvement. Therefore, this experimental study aimed to evaluate the effect of an amniotic membrane extract (AME) -loaded hydrogel on the mouse preantral follicles development. In order to have better follicle development, first, the impact of different concentrations of follicle-stimulating hormone (FSH) was evaluated on the mouse preantral follicles encapsulated in ALG. Later, the appropriate dose was adjusted for the follicles encapsulated in the ALG-AME hydrogel. Results demonstrated that 100 mIU/ml FSH showed a significant follicle survival rate compared with 10 mIU/ml FSH (P=0.005). According to MTT assay finding, the rate of weight loss, and rheology evaluations, ALG containing 1 mg/ml AME was identified as an optimal sample of follicle culture instead of other AME concentrations. Follicle diameter significantly increased in the ALG-AME 1 hydrogel compared with the ALG control group without AME (P=0.027). The storage modulus of ALG-AME 1 was 773 Pa and retained the follicle morphology for 13 days. No statistically substantial difference was seen in survival, antrum cavity formation, and competent oocyte in terms of the normal chromosomal arrangement and meiotic spindle rate in comparison with the control group. It can be concluded that ALG-AME 1 could not significantly impact the mouse preantral follicle.
基于藻酸盐（ALG）水凝胶的人工卵巢已被广泛应用于保护青春期前女性的生育能力。然而，该平台在成功模拟卵泡发育的卵巢微环境方面并不完全具备能力，仍有很大的改进潜力。因此，本实验研究旨在评估载有羊膜提取物（AME）的水凝胶对小鼠窦前卵泡发育的影响。为了实现更好的卵泡发育，首先评估了不同浓度的促卵泡激素（FSH）对包裹在ALG中的小鼠窦前卵泡的影响。随后，针对包裹在ALG-AME水凝胶中的卵泡调整了合适的剂量。结果表明，与10 mIU/ml FSH相比，100 mIU/ml FSH显示出显著更高的卵泡存活率（P = 0.005）。根据MTT测定结果、失重率和流变学评估，含有1 mg/ml AME的ALG被确定为卵泡培养的最佳样本，优于其他AME浓度。与不含AME的ALG对照组相比，ALG-AME 1水凝胶中的卵泡直径显著增加（P = 0.027）。ALG-AME 1的储能模量为773 Pa，并在13天内保持卵泡形态。与对照组相比，在正常染色体排列和减数分裂纺锤体率方面，卵泡存活率、卵泡腔形成和有能力的卵母细胞方面均未观察到统计学上的显著差异。可以得出结论，ALG-AME 1对小鼠窦前卵泡没有显著影响。
