Brito I R, Silva C M G, Duarte A B G, Lima I M T, Rodrigues G Q, Rossetto R, Sales A D, Lobo C H, Bernuci M P, Rosa-E-Silva A C J S, Campello C C, Xu M, Figueiredo J R
Faculty of Veterinary, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), PPGCV, State University of Ceará, Fortaleza, CE, Brazil.
Mol Reprod Dev. 2014 Jul;81(7):636-45. doi: 10.1002/mrd.22330. Epub 2014 Apr 25.
This study examined caprine follicular development in different concentrations of alginate matrix to determine the optimal conditions for culture. Caprine preantral follicles were cultured in a two-dimensional system (control) or a three-dimensional encapsulated system in 0.25%, 0.5%, or 1% alginate (ALG 0.25, ALG 0.5, and ALG 1, respectively). A higher percentage of morphologically normal follicles developed in ALG 0.5 and ALG 1 than in ALG 0.25 or the control (P < 0.05). The rate of antrum formation, however, was higher in ALG 0.25 than in ALG 0.5 and ALG 1 conditions (P < 0.05), but similar to the control. Follicles cultured in ALG 0.25 had higher growth rates and meiotic resumption than those cultured in ALG 0.5, ALG 1, or the control (P < 0.05). Moreover, follicles cultured in ALG 0.25 had higher levels of estradiol and progesterone than those cultured in ALG 0.5, ALG 1, or the control, as well as higher levels of CYP19A1 and HSD3B mRNA. In conclusion, a three-dimensional system that uses ALG 0.25 fosters the in vitro development of caprine preantral follicles and increases the rate of meiotic resumption.
本研究检测了不同浓度藻酸盐基质中山羊卵泡的发育情况，以确定最佳培养条件。将山羊腔前卵泡在二维系统（对照）或三维包封系统中培养，三维包封系统使用的藻酸盐浓度分别为0.25%、0.5%或1%（分别为ALG 0.25、ALG 0.5和ALG 1）。与ALG 0.25组或对照组相比，在ALG 0.5和ALG 1组中发育出的形态正常卵泡百分比更高（P < 0.05）。然而，ALG 0.25组的卵泡腔形成率高于ALG 0.5和ALG 1组（P < 0.05），但与对照组相似。在ALG 0.25中培养的卵泡比在ALG 0.5、ALG 1或对照组中培养的卵泡具有更高的生长速率和减数分裂恢复率（P < 0.05）。此外，在ALG 0.25中培养的卵泡比在ALG 0.5、ALG 1或对照组中培养的卵泡具有更高的雌二醇和孕酮水平，以及更高的CYP19A1和HSD3B mRNA水平。总之，使用ALG 0.25的三维系统促进了山羊腔前卵泡的体外发育，并提高了减数分裂恢复率。
