School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, P.R. China.
Int J Phytoremediation. 2024 Nov;26(13):2103-2112. doi: 10.1080/15226514.2024.2373974. Epub 2024 Jul 8.
Vesicular sequestration is a potential strategy for enhancing plant tolerance to cadmium (Cd) and arsenic (As). In this study, the ectopic overexpression of yeast-derived in was found to enhance the accumulation and tolerance of Cd and As in transgenic plants. ScSMF2 was localized on vacuole membranes and formed puncta structures in plant cells when agro-infiltrated for transient expression. Transgenic Arabidopsis showed less retardation on root elongation and shoot weight and more accumulation of Cd, As (III) and As (V) when cultured on medium containing Cd or As. Overexpression of promoted accumulation of Cd and arsenic in transgenic Arabidopsis, which were over twice higher than in WT plants when cultured in soil. This study provides insights into the mechanisms involved in the vesicular sequestration of heavy metals in plant and presents a potential strategy for enhancing the phytoremediation capacity of plants toward heavy metals.
液泡隔离是一种提高植物耐受镉 (Cd) 和砷 (As) 能力的潜在策略。本研究发现，酵母衍生的 ScSMF2 在 中的异位过表达可增强转基因植物对 Cd 和 As 的积累和耐受能力。ScSMF2 定位于液泡膜上，并在瞬时表达时在植物细胞中形成点状结构。当在含有 Cd 或 As 的培养基中培养时，转 ScSMF2 拟南芥的根伸长和地上部分重量的减缓程度较小，而 Cd、As (III) 和 As (V) 的积累较多。ScSMF2 的过表达促进了 Cd 和砷在转基因拟南芥中的积累，当在土壤中培养时，其积累量比 WT 植物高两倍以上。本研究深入了解了植物中重金属液泡隔离的机制，并为提高植物对重金属的植物修复能力提供了一种潜在的策略。
