在十二烷基硫酸钠溶液中，利用琼脂糖柱上的高效分子筛色谱法估算蛋白质分子量。
Estimation of protein molecular weights by high performance molecular-sieve chromatography on agarose columns in sodium dodecyl sulphate solution.
作者信息
Eriksson K O
出版信息
J Biochem Biophys Methods. 1985 Aug;11(2-3):145-52. doi: 10.1016/0165-022x(85)90050-8.
Calibration curves showing the linear relationship between -log KD and (molecular weight)2/3 are presented for proteins subjected to molecular-sieve chromatography in a 0.1% sodium dodecyl sulphate solution on crosslinked gels of different agarose concentrations (5, 9, 12 and 20%). These gels permit separation and estimation of molecular weights of proteins in the range 5000 to about 400 000, 130 000, 130 000, and 100 000, respectively. Separations of model proteins, heavy and light chains of gamma-globulin, and cyanogen bromide fragments of cellulase are shown.
给出了校准曲线，显示了在0.1%十二烷基硫酸钠溶液中，于不同琼脂糖浓度（5%、9%、12%和20%）的交联凝胶上进行分子筛色谱分析的蛋白质的-log KD与（分子量）2/3之间的线性关系。这些凝胶分别允许分离和估算分子量在5000至约400000、130000、130000和100000范围内的蛋白质。展示了模型蛋白、γ-球蛋白的重链和轻链以及纤维素酶的溴化氰片段的分离情况。
Zotero 插件