蛋白质在琼脂糖柱上的高效分子筛色谱法：凝胶浓度与孔隙率之间的关系。

High-performance molecular sieve chromatography of proteins on agarose columns: the relation between concentration and porosity of the gel.

作者信息

Hjertén S, Eriksson K O

出版信息

Anal Biochem. 1984 Mar;137(2):313-7. doi: 10.1016/0003-2697(84)90091-5.

Abstract

Curves showing the relation between log (molecular weight) and distribution coefficient are presented for proteins subjected to molecular sieve chromatography on crosslinked and non-crosslinked agarose gels of different concentrations. These curves, which facilitate selection of the gel concentration that gives optimal resolution in any particular separation problem, show that the exclusion limit of 5, 9, 12, and 20% agarose gels correspond to protein with molecular weights above 1,000,000, 600,000, 450,000, and 280,000, respectively. Plate numbers have been determined for columns of 20% agarose at different flow rates and bead sizes. Separations of model proteins by high-performance molecular sieve chromatography on agarose beads are shown.

摘要

给出了在不同浓度的交联和非交联琼脂糖凝胶上进行分子筛色谱分析的蛋白质的对数（分子量）与分配系数之间关系的曲线。这些曲线有助于选择在任何特定分离问题中能给出最佳分辨率的凝胶浓度，结果表明5%、9%、12%和20%琼脂糖凝胶的排阻极限分别对应分子量高于1000000、600000、450000和280000的蛋白质。已测定了不同流速和珠粒尺寸下20%琼脂糖柱的塔板数。展示了在琼脂糖珠上通过高效分子筛色谱对模型蛋白质的分离。