Maeda M, Ito K, Arakawa H, Tsuji A
J Immunol Methods. 1985 Sep 3;82(1):83-9. doi: 10.1016/0022-1759(85)90227-3.
We have developed a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) for thyroxine (T4) in dried blood samples spotted on filter paper. The assay is carried out on microtiter plates without extraction or centrifugation steps. The detection limit of the assay is 5 pg/disc/well, equivalent to 1.25 micrograms/1 of whole blood or 2.5 micrograms/1 of serum. Intra- and inter-assay coefficients of variation for various T4 concentrations are 2.4-9.0% and 5.9-17.5% respectively. Correlation between the proposed ELISA method and the RIA is good (r = 0.900, n = 62, y(RIA) = 0.99x(ELISA) + 9.90). The ELISA method is useful for mass-screening of neonatal congenital hypothyroidism using dried blood samples on filter paper, is very simple and one person can assay more than 300 samples per day.
我们开发了一种快速灵敏的酶联免疫吸附测定（ELISA）方法，用于检测滤纸上干燥血样中的甲状腺素（T4）。该测定在微孔板上进行，无需提取或离心步骤。该测定的检测限为5 pg/圆盘/孔，相当于全血1.25微克/升或血清2.5微克/升。不同T4浓度的批内和批间变异系数分别为2.4 - 9.0%和5.9 - 17.5%。所提出的ELISA方法与放射免疫分析（RIA）之间的相关性良好（r = 0.900，n = 62，y（RIA）= 0.99x（ELISA）+ 9.90）。ELISA方法对于使用滤纸上的干燥血样进行新生儿先天性甲状腺功能减退症的大规模筛查非常有用，非常简单，一个人每天可以检测300多个样本。
