分枝杆菌的同工酶。II. 乳酸脱氢酶酶谱在分类学和鉴定中的相关性。
Isoenzymes of mycobacteria. II. Relevance of LDH zymograms in taxonomy and identification.
作者信息
Katoch V M, Sharma V D, Datta A K, Shivannavar C T, Katoch K, Kannan K B, Bharadwaj V P
出版信息
Indian J Lepr. 1985 Jan-Mar;57(1):107-14.
The cell free extracts of mycobacteria namely M. kansasii M. avium, M. tuberculosis, BCG (Glaxo), M. gastri, M. phlei, M. smegmatis, M. vaccae, M. strain w., M. scrofulaceum, M. gordonae, M. nonchromogenicum E. coli, Staph, aureus, and M. leprae infected skin have been electrophoresed and stained for LDH activity. Normal skin tissue was also taken as control. It was found that all the organisms tested showed distinct species specific LDH isoenzyme patterns. There was no extra band but an aberrant zone of LDH activity was seen in M. leprae infected human skin in comparison to LDH isoenzymes from normal skin. No strain variations was found among the different strains of species investigated. Results described in the present paper indicate that LDH isoenzyme patterns of mycobacteria could be of identification value at species level.
对堪萨斯分枝杆菌、鸟分枝杆菌、结核分枝杆菌、卡介苗（葛兰素公司生产）、胃分枝杆菌、草分枝杆菌、耻垢分枝杆菌、母牛分枝杆菌、菌株w、瘰疬分枝杆菌、戈登分枝杆菌、非产色分枝杆菌、大肠杆菌、金黄色葡萄球菌以及麻风分枝杆菌感染的皮肤的无细胞提取物进行了电泳，并对乳酸脱氢酶（LDH）活性进行了染色。正常皮肤组织也作为对照。结果发现，所有测试的生物体都显示出独特的物种特异性LDH同工酶模式。与正常皮肤的LDH同工酶相比，在麻风分枝杆菌感染的人类皮肤中未发现额外的条带，但观察到一个异常的LDH活性区。在所研究物种的不同菌株之间未发现菌株差异。本文所述结果表明，分枝杆菌的LDH同工酶模式在物种水平上可能具有鉴别价值。
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