[用琼斯-莫特反应鉴别分枝杆菌（作者译）]

[Differentiation of mycobacteria by the Jones-Mote reaction (author's transl)].

作者信息

Kazda I

出版信息

Zentralbl Bakteriol Orig A. 1977 Feb;237(1):90-5.

Abstract

The specificity of the Jones-Mote reaction to the mycobacterial species was investigated in mycobacteria-free guinea pigs. To the sensitization and the sensitine production the following type strains (Trudeau Institute Saranac Lake) were used: M. avium, M. borstelense, M.chelonei, M. flavescens, M. fortuitum, M. gastri, M. gordonae, M.kansaii, M. marinum, M nonchromogenicum, M. phlei, M. scrofulaceum, M. smegmatis, M. terrae, M. triviale and M. bovis strain Vallee as well as M. intracellulare serotyp Davis ATCC 23435. Eight days after intracutaneous sensitization with 0,2 mg of bacterial mass (wett weight) in Al(OH)3 as adjuvans the groups of 10 animals for each species were tested intracutaneously with all 18 sensitins in a concentration of 5 mug. The animals reacted positive with the homologous sensitin only; (tab. 1) a cross-reaction was stated between M. avium and M. intracellulare (closely related) and between M. borstelense and M. chelonei (the same species). The homogeneity of the Jones-Mote reaction in some rapidly growing Mycobacteria was investigated with 11 strains of M. diernhoferi, 10 strains of M. smegmatis, M. phlei, M. fortuitum and 3 strains of M. borstelense. The positive reaction was stated with species-specific sensitin only. It should be possible to identify the primocultures using mycobacteria-free guinea pigs and suitable sensitins.

摘要

在无分枝杆菌的豚鼠中研究了琼斯-莫特反应对分枝杆菌种类的特异性。在致敏和致敏素产生过程中使用了以下标准菌株（特鲁多研究所，萨拉纳克湖）：鸟分枝杆菌、博斯特尔分枝杆菌、龟分枝杆菌、微黄分枝杆菌、偶然分枝杆菌、胃分枝杆菌、戈登分枝杆菌、堪萨斯分枝杆菌、海分枝杆菌、非产色分枝杆菌、草分枝杆菌、瘰疬分枝杆菌、耻垢分枝杆菌、土分枝杆菌、平凡分枝杆菌和牛分枝杆菌瓦利菌株，以及胞内分枝杆菌血清型戴维斯ATCC 23435。在用0.2毫克菌体质量（湿重）在氢氧化铝作为佐剂的情况下进行皮内致敏8天后，每组10只动物的每个物种用浓度为5微克的所有18种致敏素进行皮内测试。动物仅对同源致敏素呈阳性反应；（表1）在鸟分枝杆菌和胞内分枝杆菌（密切相关）之间以及博斯特尔分枝杆菌和龟分枝杆菌（同一物种）之间存在交叉反应。用11株迪尔恩霍费尔分枝杆菌、10株耻垢分枝杆菌、草分枝杆菌、偶然分枝杆菌和3株博斯特尔分枝杆菌研究了一些快速生长分枝杆菌中琼斯-莫特反应的同质性。仅用种特异性致敏素出现阳性反应。使用无分枝杆菌的豚鼠和合适的致敏素应该能够鉴定原始培养物。