An Ran, Ni Zijian, Xie Elliott, Rey Federico E, Kendziorski Christina, Thibeault Susan L
Department of Surgery, School of Medicine and Public Health (SMPH), University of Wisconsin-Madison, Madison, WI, USA.
Department of Statistics, College of Letters and Sciences , UW-Madison, Madison, WI, USA.
iScience. 2024 May 31;27(6):110156. doi: 10.1016/j.isci.2024.110156. eCollection 2024 Jun 21.
Microbiota play a critical role in the development and training of host innate and adaptive immunity. We present the cellular landscape of the upper airway, specifically the larynx, by establishing a reference single-cell atlas, while dissecting the role of microbiota in cell development and function at single-cell resolution. We highlight the larynx's cellular heterogeneity with the identification of 16 cell types and 34 distinct subclusters. Our data demonstrate that commensal microbiota have extensive impact on the laryngeal immune system by regulating cell differentiation, increasing the expression of genes associated with host defense, and altering gene regulatory networks. We uncover macrophages, innate lymphoid cells, and multiple secretory epithelial cells, whose cell proportions and expressions vary with microbial exposure. These cell types play pivotal roles in maintaining laryngeal and upper airway health and provide specific guidance into understanding the mechanism of immune system regulation by microbiota in laryngeal health and disease.
微生物群在宿主固有免疫和适应性免疫的发育与训练中发挥着关键作用。我们通过建立一个参考单细胞图谱，展示了上呼吸道，特别是喉部的细胞景观，同时以单细胞分辨率剖析微生物群在细胞发育和功能中的作用。我们通过鉴定16种细胞类型和34个不同的亚群，突出了喉部的细胞异质性。我们的数据表明，共生微生物群通过调节细胞分化、增加与宿主防御相关基因的表达以及改变基因调控网络，对喉部免疫系统产生广泛影响。我们发现了巨噬细胞、固有淋巴细胞和多种分泌性上皮细胞，它们的细胞比例和表达会随微生物暴露而变化。这些细胞类型在维持喉部和上呼吸道健康方面发挥着关键作用，并为理解微生物群在喉部健康和疾病中调节免疫系统的机制提供了具体指导。
