Lin Wenxin, Bai Mengyan, Peng Chunyan, Kuang Huaqin, Kong Fanjiang, Guan Yuefeng
Sanya Institute of China Agricultural University, Sanya, 572000 China.
College of Agronomy, China Agricultural University, Beijing, 100193 China.
aBIOTECH. 2024 May 23;5(2):196-201. doi: 10.1007/s42994-024-00158-4. eCollection 2024 Jun.
Phytic acid (PA) in grain seeds reduces the bioavailability of nutrient elements in monogastric animals, and an important objective for crop seed biofortification is to decrease the seed PA content. Here, we employed CRISPR/Cas9 to generate a PA mutant population targeting PA biosynthesis and transport genes, including two () and three (). We characterized a variety of lines containing mutations on multiple and genes. The seed PA was more significantly decreased in higher-order mutant lines with multiplex mutations. However, such mutants also exhibited poor agronomic performance. In the population, we identified two lines carrying single mutations in and , respectively. These mutants exhibited moderately reduced PA content, and regular agronomic performance compared to the wild type. Our study indicates that moderately decreasing PA by targeting single genes, rather than multiplex mutagenesis toward ultra-low PA, is an optimal strategy for low-PA soybean with a minimal trade-off in yield performance.
The online version contains supplementary material available at 10.1007/s42994-024-00158-4.
谷物种子中的植酸（PA）会降低单胃动物营养元素的生物利用率，作物种子生物强化的一个重要目标是降低种子PA含量。在此，我们利用CRISPR/Cas9技术构建了一个针对PA生物合成和转运基因的突变群体，其中包括两个（）和三个（）。我们对多种在多个和基因上存在突变的株系进行了表征。在具有多重突变的高阶突变株系中，种子PA含量下降更为显著。然而，这些突变体的农艺性状也较差。在该群体中，我们分别鉴定出两个在和中携带单突变的株系。与野生型相比，这些突变体的PA含量适度降低，且农艺性状正常。我们的研究表明，通过靶向单个基因适度降低PA，而非对超低PA进行多重诱变，是低PA大豆在产量性能上权衡最小的最优策略。
在线版本包含可在10.1007/s42994-024-00158-4获取的补充材料。
