Zhang Feng, Wang Wenlun, Yuan Anping, Li Qiong, Chu Moli, Jiang Sixia, An Yanlin
Department of Food Science and Engineering, Moutai Institute, Luban Street, Renhuai, Guizhou, China.
Department of Brewing Engineering, Moutai Institute, Luban Street, Renhuai, Guizhou, China.
Front Plant Sci. 2024 Jun 21;15:1413755. doi: 10.3389/fpls.2024.1413755. eCollection 2024.
Phosphorus deficiency and aluminum toxicity in acidic soils are important factors that limit crop yield. To further explore this issue, we identified 18 members of the gene family in the potato genome in this study. Through bioinformatics analysis, we found that the gene, an important member of this family, exhibited high expression levels in potato roots, particularly under conditions of phosphorus deficiency and aluminum toxicity stress. This suggested that the gene may play a crucial regulatory role in potato's resistance to phosphorus deficiency and aluminum toxicity. To validate this hypothesis, we conducted a series of experiments on the gene, including subcellular localization, GUS staining for tissue expression, heterologous overexpression, yeast two-hybrid hybridization, and bimolecular fluorescence complementation (BiFC). The results demonstrated that the gene is highly conserved in plants and is localized in the nucleus of potato cells. The heterologous overexpression of the gene in Arabidopsis plants resulted in a growth phenotype that exhibited resistance to both aluminum toxicity and phosphorus deficiency. Moreover, the heterologous overexpressing plants showed reduced aluminum content in the root system compared to the control group. Furthermore, we also identified an interaction between and . These results highlight the potential application of regulating the expression of the gene in potato production to enhance its adaptation to the dual stress of phosphorus deficiency and high aluminum toxicity in acidic soils.
酸性土壤中的磷缺乏和铝毒害是限制作物产量的重要因素。为了进一步探究这个问题，我们在本研究中鉴定了马铃薯基因组中该基因家族的18个成员。通过生物信息学分析，我们发现该家族的一个重要成员基因在马铃薯根中表达水平较高，尤其是在磷缺乏和铝毒害胁迫条件下。这表明该基因可能在马铃薯对磷缺乏和铝毒害的抗性中起关键调控作用。为了验证这一假设，我们对该基因进行了一系列实验，包括亚细胞定位、组织表达的GUS染色、异源过表达、酵母双杂交和双分子荧光互补（BiFC）。结果表明，该基因在植物中高度保守，定位于马铃薯细胞的细胞核。该基因在拟南芥植物中的异源过表达导致了一种对铝毒害和磷缺乏均具有抗性的生长表型。此外，与对照组相比，异源过表达植物根系中的铝含量降低。此外，我们还鉴定了与之间的相互作用。这些结果突出了调控该基因在马铃薯生产中的表达以增强其对酸性土壤中磷缺乏和高铝毒害双重胁迫适应性的潜在应用。
