Divo A A, Vande Waa J A, Campbell J R, Jensen J B
J Parasitol. 1985 Aug;71(4):504-9.
RPMI 1640 medium supplemented with adult bovine serum and hypoxanthine was superior to human serum-supplemented medium for the isolation of new strains of Plasmodium falciparum in Sudan. Similar observations in Indonesia have since confirmed our results. The chloroquine sensitivity of new isolates was identical in either human or bovine serum. Once acclimated to culture conditions P. falciparum strains grew better when using human serum. Erythrocyte-specific antibody present in adult bovine serum slightly inhibited merozoite invasion of uninfected cells. Removal of this cross-reactive antibody from bovine serum increased parasite multiplication to the level obtained in human serum.
在苏丹，添加成年牛血清和次黄嘌呤的RPMI 1640培养基，在分离恶性疟原虫新菌株方面优于添加人血清的培养基。印度尼西亚随后的类似观察结果证实了我们的研究结果。新分离株对氯喹的敏感性在人血清或牛血清中是相同的。一旦适应培养条件，恶性疟原虫菌株在使用人血清时生长得更好。成年牛血清中存在的红细胞特异性抗体轻微抑制裂殖子对未感染细胞的侵袭。从牛血清中去除这种交叉反应性抗体可使寄生虫增殖增加至人血清中的水平。
