Negahdari Nazanin, Alizadeh Sanaz, Majidi Jila, Saeed Mahdi, Ghadimi Tayyeb, Tahermanesh Kobra, Arabsorkhi-Mishabi Amirhesam, Pezeshki-Modaress Mohamad
Department of Biomedical Engineering, Islamic Azad University, Central Tehran Branch, Tehran, Iran; Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Int J Biol Macromol. 2024 Aug;275(Pt 2):133709. doi: 10.1016/j.ijbiomac.2024.133709. Epub 2024 Jul 6.
Fabrication of Core-shell nanofibrous mat which is a promising tool for a wide range of applications in tissue engineering can be developed using water in oil (W/O) or oil in water (O/W) emulsion electrospinning. In this study, for the first time, we fabricated an O/W emulsion-based electrospun core-shell mat using polycaprolactone (PCL) as a core and the blend solution of alginate (Alg) and polyethylene oxide (PEO) as shell material. To achieve a stable core-shell mat, firstly, Alg was modified with heat treatment to decrease the molecular weight of Alg. Then, to improve the chain flexibility of Alg, PEO as a second polymer was added to facilitate its electrospinnability. The different volume ratios of O/W were then fabricated by adding PCL to the Alg-PEO solution to find an optimized emulsion solution. The morphology, swelling, and porosity of the construct were evaluated. At the same time, the mechanical characteristic of fibers was evaluated in both dry and wet conditions. This study also examined cell-scaffold interactions to address the need for a scaffolding material to be suitable for tissue engineering and biomedical applications. Finally, the result exhibited a distinct core-shell structure with better mechanical properties compared to the Alg-PEO.
核壳纳米纤维垫的制备是一种在组织工程领域具有广泛应用前景的工具，可通过油包水（W/O）或水包油（O/W）乳液静电纺丝来实现。在本研究中，我们首次使用聚己内酯（PCL）作为核，海藻酸钠（Alg）与聚环氧乙烷（PEO）的混合溶液作为壳材料，制备了基于O/W乳液的静电纺核壳垫。为了获得稳定的核壳垫，首先对Alg进行热处理以降低其分子量。然后，为了提高Alg的链柔韧性，添加PEO作为第二种聚合物以促进其静电纺丝性能。接着通过向Alg-PEO溶液中添加PCL制备不同体积比的O/W乳液，以找到优化的乳液溶液。对构建体的形态、溶胀和孔隙率进行了评估。同时，在干湿条件下对纤维的力学特性进行了评估。本研究还考察了细胞与支架的相互作用，以满足对适用于组织工程和生物医学应用的支架材料的需求。最后，结果显示出与Alg-PEO相比具有更好力学性能的独特核壳结构。
