Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Japan.
Institute of Health and Medicine, Hefei Comprehensive National Science Center, Hefei, China.
Methods Mol Biol. 2024;2830:27-34. doi: 10.1007/978-1-0716-3965-8_3.
Germination test is fundamental and commonly used technique for seed dormancy and germination studies, and proper assessment of dormancy level and germination ability of a given set of seeds is prerequisite for most of the studies. However, germination is very sensitive to imbibition conditions, and dormancy development is also sensitive to growth conditions of the mother plants. In this chapter, we describe tips for plant growth and germination test mainly for physiological and molecular genetic studies with Arabidopsis. This protocol can be applied for other plant species with relatively small seeds and for various studies to analyze the effect of light, phytohormones, and other chemicals in seed germination.
萌发试验是研究种子休眠和萌发的基本且常用的技术，对特定种子群体休眠水平和萌发能力的正确评估是大多数研究的前提条件。然而，萌发对吸胀条件非常敏感，而休眠的发展也对母株的生长条件敏感。在本章中，我们描述了用于拟南芥生理和分子遗传学研究的植物生长和萌发试验的要点。该方案可应用于具有相对较小种子的其他植物物种，以及用于分析光照、植物激素和其他化学物质对种子萌发影响的各种研究。
