Institut Agro, University of Angers, INRAE, IRHS, SFR QUASAV, Angers, France.
Methods Mol Biol. 2024;2830:63-69. doi: 10.1007/978-1-0716-3965-8_6.
The longevity of seeds, also known as storability, is the period of time for which a seed lot maintains its viability during storage. The method aims to determine longevity of a seed lot during storage in a controlled environment. Seeds are first rehydrated to a preset water content (or relative humidity, RH) and then incubated under controlled conditions for various periods of time to allow for deterioration to occur. At increasing intervals during storage, seeds are retrieved and viability is tested by scoring germination of the seed lot (i.e., radicle protrusion). From these data, a survival curve can be drawn depicting loss of germination during time of storage from which different parameters estimating longevity can be inferred. These parameters can be used to compare longevity between different seed lots, genotypes, or species at similar storage conditions. This test can also be used as a proxy to measure seed vigor or physiological seed quality.
种子的寿命，也称为贮藏寿命，是指种子批在贮藏期间保持活力的时间。该方法旨在确定种子批在控制环境中的贮藏寿命。种子首先被复水至预设的水分含量（或相对湿度，RH），然后在控制条件下孵育不同的时间，以允许发生劣变。在贮藏过程中，每隔一定时间，就会取出种子并通过对种子批的发芽情况进行评分（即胚根伸出）来测试其活力。根据这些数据，可以绘制出存活曲线，描绘出贮藏过程中发芽率的损失情况，从而推断出不同的参数来估计寿命。这些参数可用于比较不同种子批、基因型或在相似贮藏条件下的不同物种之间的寿命。该测试还可以用作测量种子活力或生理种子质量的替代方法。
