Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan.
Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Methods Mol Biol. 2024;2830:131-136. doi: 10.1007/978-1-0716-3965-8_12.
Seed dormancy genes typically suppress germination and cell division. Therefore, overexpressing these genes can negatively affect tissue culture, interfering with the generation of transgenic plants and thus hampering the analysis of gene function. Transient expression in target cells is a useful approach for studying the function of seed dormancy genes. Here, we describe a protocol for transiently expressing genes related to seed dormancy in the scutellum of immature wheat (Triticum aestivum) embryos to analyze their effects on germination.
休眠种子基因通常会抑制种子的萌发和细胞分裂。因此，过度表达这些基因可能会对组织培养产生负面影响，干扰转基因植物的产生，从而阻碍对基因功能的分析。在靶细胞中进行瞬时表达是研究休眠种子基因功能的一种有用方法。本文描述了一种在未成熟小麦（Triticum aestivum）胚胎盾片中瞬时表达与种子休眠相关基因的方法，以分析它们对种子萌发的影响。
