Department of Ophthalmology, Glick Eye Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
Stark Neuroscience Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
Methods Mol Biol. 2024;2816:145-149. doi: 10.1007/978-1-0716-3902-3_14.
Clusterin, also known as apolipoprotein J, is an ATP-independent holdase chaperone protein. Clusterin is involved in various functions including protein quality control and lipid transport. Though clusterin is secreted upon stress, the intracellular fate of clusterin after a stress response is not well understood. The protocol described here utilizes clusterin tagged to fluorescent proteins like green fluorescent protein and red fluorescent protein to understand the intracellular fate of clusterin.
簇集蛋白，又被称为载脂蛋白 J，是一种 ATP 非依赖性持家伴侣蛋白。簇集蛋白参与多种功能，包括蛋白质质量控制和脂质运输。尽管簇集蛋白在应激时被分泌，但应激反应后其在细胞内的命运尚不清楚。本文描述的方案利用荧光蛋白（如绿色荧光蛋白和红色荧光蛋白）标记的簇集蛋白来了解簇集蛋白的细胞内命运。
