State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
Chengdu Agricultural College, Chengdu, 611130, Sichuan, China.
BMC Genomics. 2024 Jul 8;25(1):675. doi: 10.1186/s12864-024-10540-7.
The number of spikelets per spike is a key trait that affects the yield of bread wheat (Triticum aestivum L.). Identification of the QTL for spikelets per spike and its genetic effects that could be used in molecular assistant breeding in the future.
In this study, four recombinant inbred line (RIL) populations were generated and used, having YuPi branching wheat (YP), with Supernumerary Spikelets (SS) phenotype, as a common parent. QTL (QSS.sicau-2 A and QSS.sicau-2D) related to SS trait were mapped on chromosomes 2 A and 2D through bulked segregant exome sequencing (BSE-Seq). Fourteen molecular markers were further developed within the localization interval, and QSS.sicau-2 A was narrowed to 3.0 cM covering 7.6 Mb physical region of the reference genome, explaining 13.7 - 15.9% the phenotypic variance. Similarly, the QSS.sicau-2D was narrowed to 1.8 cM covering 2.4 Mb physical region of the reference genome, and it explained 27.4 - 32.9% the phenotypic variance. These two QTL were validated in three different genetic backgrounds using the linked markers. QSS.sicau-2 A was identified as WFZP-A, and QSS.sicau-2D was identified a novel locus, different to the previously identified WFZP-D. Based on the gene expression patterns, gene annotation and sequence analysis, TraesCS2D03G0260700 was predicted to be a potential candidate gene for QSS.sicau-2D.
Two significant QTL for SS, namely QSS.sicau-2 A and QSS.sicau-2D were identified in multiple environments were identified and their effect in diverse genetic populations was assessed. QSS.sicau-2D is a novel QTL associated with the SS trait, with TraesCS2D03G0260700 predicted as its candidate gene.
小穗数是影响面包小麦（Triticum aestivum L.）产量的关键性状。鉴定小穗数的 QTL 及其遗传效应，可用于未来的分子辅助育种。
本研究以具有多小穗表型的余皮分枝小麦（YP）为共同亲本，构建了四个重组自交系（RIL）群体。通过 bulked segregant exome sequencing（BSE-Seq），在染色体 2A 和 2D 上定位到与 SS 性状相关的 QTL（QSS.sicau-2A 和 QSS.sicau-2D）。在定位区间内进一步开发了 14 个分子标记，将 QSS.sicau-2A 缩小到 3.0 cM，覆盖参考基因组的 7.6 Mb 物理区域，解释了 13.7-15.9%的表型方差。同样，QSS.sicau-2D 缩小到 1.8 cM，覆盖参考基因组的 2.4 Mb 物理区域，解释了 27.4-32.9%的表型方差。这两个 QTL 在三个不同的遗传背景下使用连锁标记进行了验证。QSS.sicau-2A 被鉴定为 WFZP-A，QSS.sicau-2D 被鉴定为一个新的基因座，不同于先前鉴定的 WFZP-D。基于基因表达模式、基因注释和序列分析，预测 TraesCS2D03G0260700 是 QSS.sicau-2D 的潜在候选基因。
在多个环境中鉴定到两个与 SS 显著相关的 QTL，即 QSS.sicau-2A 和 QSS.sicau-2D，并评估了它们在不同遗传群体中的效应。QSS.sicau-2D 是一个与 SS 性状相关的新 QTL，预测 TraesCS2D03G0260700 是其候选基因。
