Tchakal-Mesbahi A, Metref M
Department of Cellular and Molecular Biology, University of Sciences and Technology Houari Boumdiene, Algiers, Algeria.
Burns Center, The Military Hospital M.S Neckkache, Algiers, Algeria.
Ann Burns Fire Disasters. 2024 Jun 30;37(2):140-142. eCollection 2024 Jun.
is an opportunistic pathogen rarely responsible for human infection. However, it has been reported that it causes skin and soft tissue infections and bacteremia in immune-compromised patients, such as cellulitis, abscesses, bacteremia, and wound infection. It is an oxidase and catalase-positive non-fermenter gram-negative rod that produces hydrogen sulfide. We report the case of a 90-year-old woman, who presented an invasive infectious burn wound associated with bacteremia. She was admitted into the burn center of the military hospital M.S Nekkache of Algiers, suffering from 40% TBSA with a history of diabetes. After one week of admission, the patient complained of a high fever. Microbiological culture of the catheter tip was positive and showed pale colonies on the MacConkey agar, non-lactose fermenting plate. Nutritive agar medium culture showed red pale tan colonies with a concentration >10 CFU. Identification and antibiotic susceptibility were obtained by the Phoenix system (Becton-Dickinson, USA) as This was confirmed by standards and semi-automated microbiological techniques. Gram stain showed Gram-negative bacilli with positive oxidase and catalase reactions. Production of hydrogen sulfide was confirmed by the semi-automated API 20NE method (biomerieux, France). The isolate was resistant to gentamicin, amikacin, ceftazidime, aztreonam, amoxicillin- clavulanic acid, cefepime, trimethoprim/sulfamethoxazole, and nitrofurantoin. In our case, was found in a mixed culture with No earlier exposure of the patient to marine water had been noticed. Blood culture indicated colonies growth of No further isolation of this bacteria was noticed after treatment. The patient was given imipenem, vancomycin and colistin. Despite our best efforts, the patient could not be saved because of sepsis and renal function failure.
是一种机会致病菌,很少引起人类感染。然而,据报道它可导致免疫功能低下患者发生皮肤和软组织感染及菌血症,如蜂窝织炎、脓肿、菌血症和伤口感染。它是一种氧化酶和过氧化氢酶阳性的非发酵革兰氏阴性杆菌,能产生硫化氢。我们报告一例90岁女性病例,该患者出现与菌血症相关的侵袭性感染性烧伤创面。她被收治入阿尔及尔军事医院M.S Nekkache烧伤中心,烧伤面积达40%体表面积,有糖尿病史。入院一周后,患者主诉高热。导管尖端的微生物培养呈阳性,在麦康凯琼脂非乳糖发酵平板上显示出灰白色菌落。营养琼脂培养基培养显示出浓度>10 CFU的淡红棕色菌落。通过Phoenix系统(美国BD公司)进行鉴定和药敏试验,结果为 ,这通过标准和半自动微生物技术得到了证实。革兰氏染色显示革兰氏阴性杆菌,氧化酶和过氧化氢酶反应阳性。通过半自动API 20NE方法(法国生物梅里埃公司)证实了硫化氢的产生。该分离株对庆大霉素、阿米卡星、头孢他啶、氨曲南、阿莫西林-克拉维酸、头孢吡肟、甲氧苄啶/磺胺甲恶唑和呋喃妥因耐药。在我们的病例中, 在与 的混合培养物中被发现。未发现患者此前有海水接触史。血培养显示 菌落生长。治疗后未再发现该细菌的进一步分离。患者接受了亚胺培南、万古霉素和黏菌素治疗。尽管我们尽了最大努力,但患者因败血症和肾功能衰竭未能挽救过来。