Department of Life Sciences and Health, Oslo Metropolitan University, Oslo, Norway.
Division of Medicine, Department of Gastroenterology, Akershus University Hospital, Lørenskog, Norway.
Front Immunol. 2024 Jun 24;15:1407995. doi: 10.3389/fimmu.2024.1407995. eCollection 2024.
Colorectal cancer (CRC) is a significant health issue, with notable incidence rates in Norway. The immune response plays a dual role in CRC, offering both protective effects and promoting tumor growth. This research aims to provide a detailed screening of immune-related genes and identify specific genes in CRC and adenomatous polyps within the Norwegian population, potentially serving as detection biomarkers.
The study involved 69 patients (228 biopsies) undergoing colonoscopy, divided into CRC, adenomatous polyps, and control groups. We examined the expression of 579 immune genes through nCounter analysis emphasizing differential expression in tumor versus adjacent non-tumorous tissue and performed quantitative reverse transcription polymerase chain reaction (RT-qPCR) across patient categories.
Key findings include the elevated expression of CXCL1, CXCL2, IL1B, IL6, CXCL8 (IL8), PTGS2, and SPP1 in CRC tissues. Additionally, CXCL1, CXCL2, IL6, CXCL8, and PTGS2 showed significant expression changes in adenomatous polyps, suggesting their early involvement in carcinogenesis.
This study uncovers a distinctive immunological signature in colorectal neoplasia among Norwegians, highlighting CXCL1, CXCL2, IL1B, IL6, CXCL8, PTGS2, and SPP1 as potential CRC biomarkers. These findings warrant further research to confirm their role and explore their utility in non-invasive screening strategies.
结直肠癌（CRC）是一个严重的健康问题，在挪威有显著的发病率。免疫反应在 CRC 中发挥双重作用，既提供保护作用，又促进肿瘤生长。本研究旨在对免疫相关基因进行详细筛选，并确定挪威人群中 CRC 和腺瘤性息肉中的特定基因，这些基因可能作为检测生物标志物。
该研究纳入了 69 名（228 个活检）接受结肠镜检查的患者，分为 CRC、腺瘤性息肉和对照组。我们通过 nCounter 分析检测了 579 个免疫基因的表达情况，重点分析了肿瘤与相邻非肿瘤组织之间的差异表达，并对患者进行了定量逆转录聚合酶链反应（RT-qPCR）检测。
主要发现包括 CRC 组织中 CXCL1、CXCL2、IL1B、IL6、CXCL8（IL8）、PTGS2 和 SPP1 的表达升高。此外，CXCL1、CXCL2、IL6、CXCL8 和 PTGS2 在腺瘤性息肉中也表现出显著的表达变化，提示它们在癌发生过程中的早期参与。
本研究揭示了挪威人群中结直肠肿瘤独特的免疫学特征，突出了 CXCL1、CXCL2、IL1B、IL6、CXCL8、PTGS2 和 SPP1 作为潜在的 CRC 生物标志物。这些发现需要进一步研究来确认它们的作用，并探讨它们在非侵入性筛查策略中的应用。
