Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Sci Data. 2024 Jul 9;11(1):747. doi: 10.1038/s41597-024-03612-6.
Infertility, a worldwide reproductive health concern, impacts approximately one in five couples. Male infertility, stemming from spermatogenic dysfunction and reduced sperm quality, stands as a primary factor contributing to infertility. Given the global decrease in male fertility linked to environmental factors like the greenhouse effect, it is crucial to develop a comprehensive understanding of how increased temperatures impact both the quantity and quality of sperm. In this study, we utilized Pandora-seq technology to detect the small non-coding RNAs (sncRNAs) expression profile in the testicular tissue of heat-stressed mice. The investigation explores the dynamic shifts in sncRNAs within the mouse testis under heat stress, including miRNAs, tsRNAs, piRNAs, rsRNAs and other sncRNAs. Furthermore, we successfully identified differentially expressed sncRNAs in testicular tissues before and after heat stress. Subsequently, we conducted functional enrichment analysis on the potential predicted target genes of differentially expressed miRNAs and tsRNAs. These datasets will constitute a valuable foundational resource for further investigations into the decline in male reproductive capacity triggered by heat stress.
不育症是一个全球性的生殖健康问题，大约影响五分之一的夫妇。男性不育症源于精子发生功能障碍和精子质量下降，是导致不育症的主要因素。鉴于与温室效应等环境因素相关的全球男性生育力下降，了解高温如何影响精子的数量和质量至关重要。在这项研究中，我们利用 Pandora-seq 技术检测了热应激小鼠睾丸组织中的小非编码 RNA（sncRNA）表达谱。该研究探讨了热应激下小鼠睾丸内 sncRNA 的动态变化，包括 miRNA、tsRNA、piRNA、rsRNA 和其他 sncRNA。此外，我们成功鉴定了热应激前后睾丸组织中差异表达的 sncRNA。随后，我们对差异表达 miRNA 和 tsRNA 的潜在预测靶基因进行了功能富集分析。这些数据集将为进一步研究热应激引起的男性生殖能力下降提供有价值的基础资源。
