泛癌症分析及 GPC1 在肝细胞癌中的致癌作用。

Pan-cancer analysis and the oncogenic role of Glypican 1 in hepatocellular carcinoma.

机构信息

Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.

Biomedical Experimental Center of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.

出版信息

Sci Rep. 2024 Jul 9;14(1):15870. doi: 10.1038/s41598-024-66838-9.

DOI:10.1038/s41598-024-66838-9

PMID:38982153

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11233571/

Abstract

Recent studies indicate that Glypican 1 (GPC-1) is aberrantly expressed and plays a key role in certain cancers, but little is known in the hepatocellular carcinoma. Raw data from TCGA, GTEx and TIMER databases were utilized to comprehensively analyze GPC-1 expression landscape in pan-cancer, and the biological function of GPC-1 was investigated in liver cancer cells. The results revealed that GPC-1 is highly expressed in HCC, negatively correlated with survival, and also positively correlated with immune infiltration and clinical stage. Furthermore, GPC-1 promoted cell proliferation and inhibited apoptosis in the HCC cell lines. WGCNA analysis and HCCDB database revealed that Akt acted as a key molecule related to GPC-1, influencing biological functions and regulating cell malignant behaviors via the AKT signaling pathway. In conclusion, our findings provide a relatively comprehensive understanding of the oncogenic role of GPC-1 in HCC, implying that GPC-1 could serve as an innovative therapeutic target.

摘要

最近的研究表明，Glypican 1（GPC-1）表达异常，在某些癌症中发挥关键作用，但在肝细胞癌中知之甚少。我们利用 TCGA、GTEx 和 TIMER 数据库中的原始数据，全面分析了 GPC-1 在泛癌症中的表达图谱，并在肝癌细胞中研究了 GPC-1 的生物学功能。结果表明，GPC-1 在 HCC 中高表达，与生存呈负相关，与免疫浸润和临床分期呈正相关。此外，GPC-1 促进 HCC 细胞系的细胞增殖和抑制细胞凋亡。WGCNA 分析和 HCCDB 数据库表明，Akt 作为与 GPC-1 相关的关键分子，通过 AKT 信号通路影响生物功能并调节细胞恶性行为。总之，我们的研究结果提供了对 GPC-1 在 HCC 中致癌作用的相对全面的理解，表明 GPC-1 可能成为一种创新的治疗靶点。