Laboratório de Cultura de Tecidos-LCT, Instituto de Biotecnologia Aplicada à Agropecuária-BIOAGRO, Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Laboratório de Genética e Biotecnologia, ICB, Departamento de Biologia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
Methods Mol Biol. 2024;2827:223-241. doi: 10.1007/978-1-0716-3954-2_16.
Over the years, our team has dedicated significant efforts to studying a unique natural dye-producing species, annatto (Bixa orellana L.). We have amassed knowledge and established foundations that support the applications of gene expression analysis in comprehending in vitro morphogenic regeneration processes, phase transition aspects, and bixin biosynthesis. Additionally, we have conducted gene editing associated with these processes. The advancements in this field are expected to enhance breeding practices and contribute to the overall improvement of this significant woody species. Here, we present a step-by-step protocol based on somatic embryogenesis and an optimized transformation protocol utilizing Agrobacterium tumefaciens.
多年来，我们的团队致力于研究一种独特的天然染料生产物种——胭脂树（Bixa orellana L.）。我们积累了知识并建立了基础，支持利用基因表达分析来理解体外形态发生再生过程、相变方面和胭脂树红生物合成。此外，我们还进行了与这些过程相关的基因编辑。预计该领域的进展将增强育种实践，并为这个重要的木本物种的整体改善做出贡献。在这里，我们提出了一个基于体细胞胚胎发生的分步方案和一个利用根癌农杆菌的优化转化方案。
